CCL24/Eotaxin 2 Polyclonal antibody

CCL24/Eotaxin 2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 22306-1-AP

Synonyms

CCL24, Eotaxin 2, CK-beta-6, CCL-24, CCL 24

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Validation Data Gallery

• 

  WB analysis of mouse thymus using 22306-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22306-1-AP (CCL24/Eotaxin 2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 22306-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22306-1-AP (CCL24/Eotaxin 2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22306-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22306-1-AP (CCL24/Eotaxin 2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22306-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22306-1-AP (CCL24/Eotaxin 2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 22306-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22306-1-AP (CCL24/Eotaxin 2 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse thymus tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

22306-1-AP targets CCL24/Eotaxin 2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: chemokine (C-C motif) ligand 24
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: NM_002991
• Gene Symbol: CCL24/Eotaxin 2
• Gene ID (NCBI): 6369
• RRID: AB_2879068
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chemokine CCL24 is the second member of eotaxins, a group of eosinophils' selectively chemoattractants. CCL24 is constitutively expressed in fibroblasts, epithelial cells, macrophages. CCL24 has only one receptor CCR3, which mainly presents on eosinophils and was also found on basophils, monocytes, Th2 lymphocytes, epithelial cells and airway smooth muscles. CCL24 displays chemotactic activity on resting T lymphocytes, a minimal activity on neutrophils, and is negative on monocytes and activated T lymphocytes.

Publications

Species	Application	Title
mouse	WB	Dis Markers; CCL24 Protects Renal Function by Controlling Inflammation in Podocytes.; Authors - Youdi Wang
human,mouse	IHC,IF	Cell Death Dis; Mechanical stiffness promotes skin fibrosis via Piezo1-Wnt2/Wnt11-CCL24 positive feedback loop; Authors - Jiahao He