Cyclin B1 Antibody

Cyclin B1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 55004-1-AP

Synonyms

CCNB, CCNB1, cyclin B1, G2/mitotic specific cyclin B1



Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, PC-3 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

55004-1-AP targets Cyclin B1 in IP, WB, ELISA applications and shows reactivity with human, mouse samples.

Reactivity human, mouse
Cited Species human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cyclin B1
Calculated molecular weight 48 kDa
Observed molecular weight 55-60 kDa
GenBank accession number NM_031966
Gene symbol CCNB1
Gene ID (NCBI) 891
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

Protocols

Product Specific Protocols
WB protocol for Cyclin B1 antibody 55004-1-APDownload protocol
IP protocol for Cyclin B1 antibody 55004-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Aging (Albany NY)

The ubiquitin ligase KBTBD8 regulates PKM1 levels via Erk1/2 and Aurora A to ensure oocyte quality.

Authors - Yan-Ru Li
humanWB

Cancers (Basel)

Metformin Treatment Suppresses Melanoma Cell Growth and Motility Through Modulation of microRNA Expression.

Authors - Hui-Wen Tseng
humanWB

Biomed Pharmacother

Lin28 promotes dental pulp cell proliferation via upregulation of cyclin-dependent proteins and interaction with let-7a/IGF2BP2 pathways.

Authors - Yan Liu
humanWB

Sci Rep

Low C6orf141 Expression is Significantly Associated with a Poor Prognosis in Patients with Oral Cancer.

Authors - Cheng-Mei Yang
humanWB,IHC

Onco Targets Ther

Prognostic implications and oncogenic roles of MYBL2 protein expression in esophageal squamous-cell carcinoma.

Authors - Hui Qin
humanWB

J Exp Clin Cancer Res

Nuciferine inhibits the progression of glioblastoma by suppressing the SOX2-AKT/STAT3-Slug signaling pathway.

Authors - Zizhuo Li