Name: Cyclin B1 Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 55004-1-AP

Tested Applications

Applications:

WB, IP, IF, ELISA
Application not tested? Please see our Guarantee

Cyclin B1 Antibody WB HepG2 cells 55004-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 55004-1-AP(CCNB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Cyclin B1 Antibody IP HeLa cells 55004-1-AP

IP Result of anti-Cyclin B1 (IP:55004-1-AP, 4ug; Detection:55004-1-AP 1:500) with HeLa cells lysate 1200ug.

Species specificity:

human, mouse, rat

Positive WB detected in:

HepG2 cells, PC-3 cells, HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells

Positive IP detected in:

HeLa cells

Recommended dilution:

WB : 1:500-1:3000

IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

Sample-dependent, check data in Validation tab

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

Peptide

Full name:

cyclin B1

Calculated molecular weight:

48 kDa

Observed molecular weight:

55-60 kDa

GenBank accession number:

NM_031966

Gene ID (NCBI):

891

Gene symbol

CCNB1

Synonyms

CCNB, CCNB1, cyclin B1, G2/mitotic specific cyclin B1

Background

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

Showing 1- [6] of [78] Publications

Author:		Zheng Lei L
Pubmed ID:		23688861
Journal:		Food Chem Toxicol
Title:		Brucine, an effective natural compound derived from nux-vomica, induces G1 phase arrest and apoptosis in LoVo cells.
Application:		WB

Species:		human

Author:		Xianfang Meng
Pubmed ID:		24444371
Journal:		J Cell Mol Med
Title:		Protection of neurons from high glucose-induced injury by deletion of MAD2B.
Application:		IF

Species:		rat

Author:		Hao Chen
Pubmed ID:		24420967
Journal:		Proteomics
Title:		iTRAQ-based proteomic analysis of dioscin on human HCT-116 colon cancer cells.
Application:		WB

Species:		human

Author:		Zhen Liu
Pubmed ID:		24814201
Journal:		J Surg Res
Title:		The involvement of CXCR7 in modulating the progression of papillary thyroid carcinoma.
Application:		WB

Species:		human

Author:		Bingling Dai
Pubmed ID:		24814261
Journal:		Environ Toxicol Pharmacol
Title:		Eupolyphaga sinensis Walker inhibits human chronic myeloid leukemia cell K562 growth by inducing G2-M phase cell cycle arrest and targeting EGFR signaling pathway and in S180 tumor-bearing mice.
Application:		WB

Species:		human

Author:		Yanmin Zhang
Pubmed ID:		24980220
Journal:		Sci Rep
Title:		Eupolyphaga sinensis Walker displays inhibition on hepatocellular carcinoma through regulating cell growth and metastasis signaling.
Application:		WB

Species:		human

WB Figures

Cyclin B1 Antibody WB HeLa cells 55004-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 55004-1-AP( Cyclin B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

HepG2 cells were subjected to SDS PAGE followed by western blot with 55004-1-AP(CCNB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Cyclin B1 Antibody WB Jurkat cells 55004-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 55004-1-AP(CCNB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 55004-1-AP( Cyclin B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Cyclin B1 Antibody WB K-562 cells 55004-1-AP

K-562 cells were subjected to SDS PAGE followed by western blot with 55004-1-AP(CCNB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Cyclin B1 Antibody WB NIH/3T3 cells 55004-1-AP

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Cyclin B1 Antibody WB PC-3 cells 55004-1-AP

PC-3 cells were subjected to SDS PAGE followed by western blot with 55004-1-AP(CCNB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

IP Figures

IP Result of anti-Cyclin B1 (IP:55004-1-AP, 4ug; Detection:55004-1-AP 1:500) with HeLa cells lysate 1200ug.

Cyclin B1 Antibody 78 Publications

Rabbit Polyclonal| Catalog number: 55004-1-AP

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Tested Applications

Product Information

Immunogen Information

Background

Showing 1- [6] of [78] Publications

WB Figures

IP Figures

Product-specific protocols

Standard Protocols

Cyclin B1 Antibody 78 Publications

Rabbit Polyclonal| Catalog number: 55004-1-AP

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Tested Applications

Product Information

Immunogen Information

Background