Cyclin B1 Polyclonal antibody
Cyclin B1 Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IF,ELISA
Conjugate
Unconjugated
124
Cat no : 55004-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, NIH/3T3 cells, PC-3 cells, HepG2 cells, Jurkat cells, K-562 cells |
Positive IP detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 116 publications below |
IHC | See 2 publications below |
IF | See 1 publications below |
Product Information
55004-1-AP targets Cyclin B1 in WB, IP, IF,ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | cyclin B1 |
Calculated molecular weight | 48 kDa |
Observed molecular weight | 55-60 kDa |
GenBank accession number | NM_031966 |
Gene symbol | CCNB1 |
Gene ID (NCBI) | 891 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.
Protocols
Product Specific Protocols | |
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WB protocol for Cyclin B1 antibody 55004-1-AP | Download protocol |
IP protocol for Cyclin B1 antibody 55004-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Aging (Albany NY) The ubiquitin ligase KBTBD8 regulates PKM1 levels via Erk1/2 and Aurora A to ensure oocyte quality. | ||
Biomed Pharmacother Lin28 promotes dental pulp cell proliferation via upregulation of cyclin-dependent proteins and interaction with let-7a/IGF2BP2 pathways. | ||
Sci Rep Low C6orf141 Expression is Significantly Associated with a Poor Prognosis in Patients with Oral Cancer. | ||
Onco Targets Ther Prognostic implications and oncogenic roles of MYBL2 protein expression in esophageal squamous-cell carcinoma. | ||
J Exp Clin Cancer Res Nuciferine inhibits the progression of glioblastoma by suppressing the SOX2-AKT/STAT3-Slug signaling pathway. |