Cyclin B1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Cyclin B1 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IP, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

206

Cat no : 55004-1-AP

Synonyms

CCNB, CCNB1, cyclin B1, G2/mitotic specific cyclin B1

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Validation Data Gallery

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  WB analysis using 55004-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55004-1-AP

  WB result of Cyclin B1 antibody (55004-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B1 transfected HeLa cells.

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  WB analysis of HeLa using 55004-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55004-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 55004-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (CCNB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 55004-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 55004-1-AP

  IP Result of anti-Cyclin B1 (IP:55004-1-AP, 4ug; Detection:55004-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IF Staining of HeLa using 55004-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin B1 antibody (55004-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Jurkat using 55004-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human Cyclin B1 (55004-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, PC-3 cells, Jurkat cells, K-562 cells
• Positive IP detected in: HeLa cells
• Positive IF detected in: HeLa cells
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 196 publications below
• IHC: See 7 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

55004-1-AP targets Cyclin B1 in WB, IP, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, bovine, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cyclin B1
• Calculated molecular weight: 48 kDa
• Observed molecular weight: 55-60 kDa
• GenBank accession number: NM_031966
• Gene symbol: CCNB1
• Gene ID (NCBI): 891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

Publications

Species	Application	Title
human	WB	Nat Commun; Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.; Authors - Xiaoyu Ma
human	WB	Autophagy; PRCC-TFE3 fusion-mediated PRKN/parkin-dependent mitophagy promotes cell survival and proliferation in PRCC-TFE3 translocation renal cell carcinoma.; Authors - Bo Wang
human,mouse	WB	Cell Death Dis; HDAC7/c-Myc signaling pathway promotes the proliferation and metastasis of choroidal melanoma cells; Authors - Yimeng Zhang
mouse	WB	Kidney Int; Myeloid-derived Growth Factor Deficiency Exacerbates Mitotic Catastrophe of Podocytes in Glomerular Disease.; Authors - Ping Zhan
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song
human	WB	Cell Rep; Loss of Brap Results in Premature G1/S Phase Transition and Impeded Neural Progenitor Differentiation.; Authors - Alison A Lanctot

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