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Cyclin B1 Antibody 81 Publications

Rabbit Polyclonal| Catalog number: 55004-1-AP

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Con: 52 μg/150 μl

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Tested Applications

Applications:
WB, IP, IF, ELISA
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Images See all

Cyclin B1 Antibody WB HeLa cells 55004-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

Cyclin B1 Antibody IP HeLa cells 55004-1-AP

IP Result of anti-Cyclin B1 (IP:55004-1-AP, 4ug; Detection:55004-1-AP 1:500) with HeLa cells lysate 1200ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells, NIH/3T3 cells, PC-3 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IP detected in:
HeLa cells
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
cyclin B1
Calculated molecular weight:
48 kDa
Observed molecular weight:
55-60 kDa
GenBank accession number:
NM_031966
Gene ID (NCBI):
891
Gene symbol
CCNB1
Synonyms
CCNB, CCNB1, cyclin B1, G2/mitotic specific cyclin B1

Background

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.


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