Cyclin B1 Antibody
Cyclin B1 Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
IP, WB, ELISA
Conjugate
Unconjugated
100
Cat no : 55004-1-AP
Synonyms
CCNB, CCNB1, cyclin B1, G2/mitotic specific cyclin B1
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, NIH/3T3 cells, PC-3 cells, HepG2 cells, Jurkat cells, K-562 cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 92 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
55004-1-AP targets Cyclin B1 in IP, WB, ELISA applications and shows reactivity with human, mouse samples.
| Reactivity | human, mouse |
| Cited Species | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | cyclin B1 |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 55-60 kDa |
| GenBank accession number | NM_031966 |
| Gene symbol | CCNB1 |
| Gene ID (NCBI) | 891 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20oC. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cyclin B1 antibody 55004-1-AP | Download protocol |
| IP protocol for Cyclin B1 antibody 55004-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) The ubiquitin ligase KBTBD8 regulates PKM1 levels via Erk1/2 and Aurora A to ensure oocyte quality. | ||
Cancers (Basel) Metformin Treatment Suppresses Melanoma Cell Growth and Motility Through Modulation of microRNA Expression. | ||
Biomed Pharmacother Lin28 promotes dental pulp cell proliferation via upregulation of cyclin-dependent proteins and interaction with let-7a/IGF2BP2 pathways. | ||
Sci Rep Low C6orf141 Expression is Significantly Associated with a Poor Prognosis in Patients with Oral Cancer. | ||
Onco Targets Ther Prognostic implications and oncogenic roles of MYBL2 protein expression in esophageal squamous-cell carcinoma. | ||
J Exp Clin Cancer Res Nuciferine inhibits the progression of glioblastoma by suppressing the SOX2-AKT/STAT3-Slug signaling pathway. |
