Cyclin D2 Monoclonal antibody

Cyclin D2 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse and More (1)

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

1A12A8

Cat no : 67048-1-Ig

Synonyms

CCND2, cyclin D, cyclin D2, G1/S specific cyclin D2, KIAK0002



Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells, U2OS cells, MCF-7 cells, Caco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

67048-1-Ig targets Cyclin D2 in WB,ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cyclin D2 fusion protein Ag25190
Full Name cyclin D2
Calculated molecular weight 32 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC010958
Gene symbol CCND2
Gene ID (NCBI) 894
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

CCND2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. CCND2 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND2 has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. High level expression of CCND2 was observed in ovarian and testicular tumors.

Protocols

Product Specific Protocols
WB protocol for Cyclin D2 antibody 67048-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Technol Cancer Res Treat

The Analysis of Differentially Expressed circRNAs Under the Antiproliferative Effect From 5-Fluorouracil on Osteosarcoma Cells.

Authors - AiJun Huang
humanWB

Cell Death Dis

Long intergenic non-coding RNA 00473 promotes proliferation and migration of gastric cancer via the miR-16-5p/CCND2 axis and by regulating AQP3.

Authors - Shuaishuai Zhuo