CCR2b-specific Polyclonal antibody

CCR2b-specific Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16154-1-AP

Synonyms

CCR2, CC-CKR-2, C-C CKR-2, CC CKR 2, C-C chemokine receptor type 2

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Validation Data Gallery

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  IHC staining of human tonsillitis using 16154-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16154-1-AP (CCR2b-specific Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 16154-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16154-1-AP (CCR2b-specific Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

16154-1-AP targets CCR2b-specific in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: chemokine (C-C motif) receptor 2
• Calculated Molecular Weight: 41 kDa
• GenBank Accession Number: NM_000648
• Gene Symbol: CCR2
• Gene ID (NCBI): 729230
• RRID: AB_2878224
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCR2 encodes a receptor for MCP1, functioning in inflammatory processes, tumor infiltration, and monocyte invasion of artery walls in atherosclerosis. There are two isoforms of CCR2, CCR2A and CCR2B. CCR2 isoforms were expressed by different cell subsets in both normal and IIM muscle. CCR2A was expressed in vessel walls and by some mononuclear cells, especially in cells involved in partial invasion in PM and IBM. CCR2B expression was observed in all satellite cells, in the muscular domain of neuromuscular junctions, and in some regenerative fibers of IIM, but not in inflammatory exudates. The sequences of the two isoforms are different from 314-374 aa. This antibody 16154-1-AP is raised against the peptide of 344-360 amino acids of CCR2b. It is specific to CCR2b.

Publications

Species	Application	Title
human	IF	Cell Rep; Tissue-resident, extravascular Ly6c- monocytes are critical for inflammation in the synovium; Authors - Anna B Montgomery
human	IF	Int J Mol Sci; Inducing Receptor Degradation as a Novel Approach to Target CC Chemokine Receptor 2 (CCR2); Authors - Natalia V Ortiz Zacarías
human	WB,IHC	Cancer Med; Combination therapy with bevacizumab and a CCR2 inhibitor for human ovarian cancer: An in vivo validation study; Authors - Tianyue Zhai