CCR2b-specific Polyclonal antibody

CCR2b-specific Polyclonal Antibody for IHC, ELISA
Cat No. 16154-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

CCR2, C C chemokine receptor type 2, C C CKR 2, C-C chemokine receptor type 2, CC CKR 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IHC detected inhuman liver cancer tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16154-1-AP targets CCR2b-specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide Predict reactive species
Full Name chemokine (C-C motif) receptor 2
Calculated Molecular Weight 41 kDa
GenBank Accession NumberNM_000648
Gene Symbol CCR2
Gene ID (NCBI) 729230
RRIDAB_2878224
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP41597
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCR2 encodes a receptor for MCP1, functioning in inflammatory processes, tumor infiltration, and monocyte invasion of artery walls in atherosclerosis. There are two isoforms of CCR2, CCR2A and CCR2B. CCR2 isoforms were expressed by different cell subsets in both normal and IIM muscle. CCR2A was expressed in vessel walls and by some mononuclear cells, especially in cells involved in partial invasion in PM and IBM. CCR2B expression was observed in all satellite cells, in the muscular domain of neuromuscular junctions, and in some regenerative fibers of IIM, but not in inflammatory exudates. The sequences of the two isoforms are different from 314-374 aa. This antibody 16154-1-AP is raised against the peptide of 344-360 amino acids of CCR2b. It is specific to CCR2b.

Protocols

Product Specific Protocols
IHC protocol for CCR2b-specific antibody 16154-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Rep

Tissue-resident, extravascular Ly6c- monocytes are critical for inflammation in the synovium

Authors - Anna B Montgomery
humanIF

Int J Mol Sci

Inducing Receptor Degradation as a Novel Approach to Target CC Chemokine Receptor 2 (CCR2)

Authors - Natalia V Ortiz Zacarías
humanWB,IHC

Cancer Med

Combination therapy with bevacizumab and a CCR2 inhibitor for human ovarian cancer: An in vivo validation study

Authors - Tianyue Zhai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Larissa (Verified Customer) (04-14-2025)

We've used the CCR2b-specific Rabbit Polyclonal Antibody for both Western blot and immunohistochemistry with great results. Overall, a reliable and high-quality antibody that performs well in multiple applications.

  • Applications: Western Blot, Immunohistochemistry
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