TCP1 eta Monoclonal antibody, PBS Only

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  WB analysis using 67540-1-Ig (same clone as 67540-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67540-1-Ig (CCT7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67540-1-Ig (same clone as 67540-1-PBS)

  WB result of CCT7 antibody (67540-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT7 transfected HEK-293 cells. This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67540-1-Ig (same clone as 67540-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67540-1-Ig (CCT7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 67540-1-Ig (same clone as 67540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67540-1-Ig (TCP1 eta antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 67540-1-Ig (same clone as 67540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67540-1-Ig (TCP1 eta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67540-1-Ig (same clone as 67540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67540-1-Ig (TCP1 eta antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 67540-1-Ig (same clone as 67540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67540-1-Ig (TCP1 eta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 67540-1-Ig (same clone as 67540-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CCT7 antibody (67540-1-Ig, Clone: 2C1G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 67540-1-Ig (same clone as 67540-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCT7 antibody (67540-1-Ig, Clone: 2C1G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

TCP1 eta Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67540-1-PBS

Clone No.2C1G1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CCT7, CCT ETA, CCT-eta, CCTH, Chaperonin containing T-complex polypeptide 1 subunit 7

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Product Information

67540-1-PBS targets TCP1 eta in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9502

  Product name: Recombinant human CCT7 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 240-543 aa of BC019296

  Sequence: LNVELELKAEKDNAEIRVHTVEDYQAIVDAEWNILYDKLEKIHHSGAKVVLSKLPIGDVATQYFADRDMFCAGRVPEEDLKRTMMACGGSIQTSVNALSADVLGRCQVFEETQIGGERYNFFTGCPKAKTCTFILRGGAEQFMEETERSLHDAIMIVRRAIKNDSVVAGGGAIEMELSKYLRDYSRTIPGKQQLLIGAYAKALEIIPRQLCDNAGFDATNILNKLRARHAQGGTWYGVDINNEDIADNFEAFVWEPAMVRINALTAASEAACLIVSVDETIKNPRSTVDAPTAAGRGRGRGRPH

• Full Name: chaperonin containing TCP1, subunit 7 (eta)
• Calculated Molecular Weight: 543 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC019296
• Gene Symbol: CCT7
• Gene ID (NCBI): 10574
• RRID: AB_2882759
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99832
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CCT7 is a subunit of CCT complex-a large cytosolic chaperonin complex composed of two hetero-oligomeric stacked rings able to interact with nascent polypeptides, which mediates protein folding in an ATP-dependent manner and prevents aggregation in eukaryotes. Each ring consists of eight different subunits (CCT1 to CCT8). CCT7 is involved in preventing aggregation of GPCRs and in regulating their expression, maturation, and transport to the cell surface.