CCT8 Monoclonal antibody

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  WB analysis using 67539-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67539-1-Ig (CCT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67539-1-Ig

  WB result of CCT8 antibody (67539-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT8 transfected HEK-293 cells.

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  WB analysis of NIH/3T3 using 67539-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67539-1-Ig (CCT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67539-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67539-1-Ig (CCT8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67539-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67539-1-Ig (CCT8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 67539-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CCT8 antibody (67539-1-Ig, Clone: 1A7C3 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• Featured Product
• KD/KO Validated

CCT8 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67539-1-Ig

Clone No.1A7C3

Publications(4)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

C21orf112, CCT theta, CCT8, Cctq, D21S246, KIAA0002, PRED71, TCP 1 theta

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Tested Applications

• Positive WB detected in: HEK-293 cells, NIH/3T3 cells, A549 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Product Information

67539-1-Ig targets CCT8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30082

  Product name: Recombinant human CCT8 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 146-497 aa of BC012584

  Sequence: FNVDNIRVCKILGSGISSSSVLHGMVFKKETEGDVTSVKDAKIAVYSCPFDGMITETKGTVLIKTAEELMNFSKGEENLMDAQVKAIADTGANVVVTGGKVADMALHYANKYNIMLVRLNSKWDLRRLCKTVGATALPRLTPPVLEEMGHCDSVYLSEVGDTQVVVFKHEKEDGAISTIVLRGSTDNLMDDIERAVDDGVNTFKVLTRDKRLVPGGGATEIELAKQITSYGETCPGLEQYAIKKFAEAFEAIPRALAENSGVKANEVISKLYAVHQEGNKNVGLDIEAEVPAVKDMLEAGILDTYLGKYWAIKLATNAAVTVLRVDQIIMAKPAGGPKPPSGKKDWDDDQND

• Full Name: chaperonin containing TCP1, subunit 8 (theta)
• Calculated Molecular Weight: 548 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC012584
• Gene Symbol: CCT8
• Gene ID (NCBI): 10694
• RRID: AB_2882758
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P50990
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCT8, also named as C21orf112, CCTQ, KIAA0002, CCT-theta, TCP-1-theta and NY-REN-15, belongs to the TCP-1 chaperonin family. It is a molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT8 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Dysregulation of CCT8 has been linked to proliferation and invasion of tumors.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; Mechanism of orphan subunit recognition during assembly quality control; Authors - Yuichi Yagita
human	WB	Cell; CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.; Authors - Xinyu Ma
human	WB,IHC,IP	J Cancer; CCT8 promotes cell migration and tumor metastasis in lung adenocarcinomas; Authors - Zhiqiang Wu
human	IHC	Medicine (Baltimore); Chaperonin-containing TCP-1 subunit genes are potential prognostic biomarkers and are correlated with Th2 cell infiltration in lung adenocarcinoma: An observational study; Authors - Ruijuan Du