Anti-Mouse CD103 (2E7) Armenian Hamster Recombinant Antibody

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  FC experiment of mouse splenocytes using 65677-2-AR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD103 (2E7) Armenian Hamster IgG2a RecAb (65677-2-AR, Clone: 2E7) or Armenian Hamster IgG Isotype Control (PIP) (65210-1-Ig, Clone: PIP), and FITC-conjugated Anti-Armenian Hamster IgG antibody. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 65677-2-AR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD103 (2E7) Armenian Hamster IgG2a RecAb (65677-2-AR, Clone: 2E7)(red) or Armenian Hamster IgG Isotype Control (PIP) (65210-1-Ig, Clone: PIP) (blue), and FITC-conjugated Anti-Armenian Hamster IgG antibody. Cells were not fixed.

CD103 Uni-rAb^® Recombinant Antibody for FC

Cat No. 65677-2-AR

Clone No.2E7

Host / Isotype

Armenian Hamster / IgG2a

Reactivity

mouse

Applications

FC

Synonyms

Itgae, alpha E1, alpha M290, aM290, Integrin alpha E

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65677-2-AR targets CD103 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Armenian Hamster / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  N/A

• Full Name: integrin alpha E, epithelial-associated
• GenBank Accession Number: BC150690
• Gene Symbol: CD103
• Gene ID (NCBI): 16407
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q60677
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD103, also known as integrin alpha-E (ITGAE) or integrin alpha-IEL, is a type I transmembrane integrin protein that binds integrin beta 7 to form Integrin alpha-E beta-7 which is a receptor for E-cadherin and mediates adhesion of T lymphocytes to epithelial cells (PMID: 7969453; 8468482). CD103 is expressed on intraepithelial lymphocyte (IEL) T cells (both alpha/beta T cells and gamma/delta T cells), some peripheral regulatory T cells (Tregs), lamina propria T cells, and a subset of dendritic cells in the gut mucosa and mesenteric lymph nodes (PMID: 12242333; 15608520; 16216890).