CD107a / LAMP1 Monoclonal antibody
CD107a / LAMP1 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
3B2E9
Cat no : 67300-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HT-1080 cells, HeLa cells, HepG2 cells, Jurkat cells, THP-1 cells, MDA-MB-231 cells, JAR cells, K-562 cells, U-937 cells |
Positive IHC detected in | human liver cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human liver cancer tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 3 publications below |
IF | See 2 publications below |
Product Information
67300-1-Ig targets CD107a / LAMP1 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD107a / LAMP1 fusion protein Ag28869 |
Full Name | lysosomal-associated membrane protein 1 |
Calculated molecular weight | 417 aa, 45 kDa |
Observed molecular weight | 90-120 kDa |
GenBank accession number | BC021288 |
Gene symbol | LAMP1 |
Gene ID (NCBI) | 3916 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CD107a / LAMP1 antibody 67300-1-Ig | Download protocol |
IHC protocol for CD107a / LAMP1 antibody 67300-1-Ig | Download protocol |
IF protocol for CD107a / LAMP1 antibody 67300-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancers (Basel) A Novel Phenazine Analog, CPUL1, Suppresses Autophagic Flux and Proliferation in Hepatocellular Carcinoma: Insight from Integrated Transcriptomic and Metabolomic Analysis | ||
Antioxidants (Basel) Stimulation of Hepatic Ferritinophagy Mitigates Irp2 Depletion-Induced Anemia | ||
Nat Commun Cholesterol removal improves performance of a model biomimetic system to co-deliver a photothermal agent and a STING agonist for cancer immunotherapy | ||
Front Immunol RAB20 deficiency promotes the development of silicosis via NLRP3 inflammasome |