CD107a Monoclonal antibody

CD107a Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

3B2E9

Cat no : 67300-1-Ig

Synonyms

CD107a, LAMP 1, LAMP1, LAMPA, LGP120



Tested Applications

Positive WB detected inHT-1080 cells, HeLa cells, HepG2 cells, Jurkat cells, THP-1 cells, MDA-MB-231 cells, JAR cells, K-562 cells, U-937 cells
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67300-1-Ig targets CD107a in WB, IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD107a fusion protein Ag28869
Full Name lysosomal-associated membrane protein 1
Calculated molecular weight 417 aa, 45 kDa
Observed molecular weight 90-120 kDa
GenBank accession numberBC021288
Gene symbol LAMP1
Gene ID (NCBI) 3916
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.

Protocols

Product Specific Protocols
WB protocol for CD107a antibody 67300-1-IgDownload protocol
IHC protocol for CD107a antibody 67300-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols