CoraLite® Plus 488-conjugated CD107a / LAMP1 Monoclonal antibody

CD107a / LAMP1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B2E9

Cat no : CL488-67300

Synonyms

CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker

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Validation Data Gallery

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  IF Staining of human liver cancer using CL488-67300

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 CD107a antibody (CL488-67300, Clone: 3B2E9 ) at dilution of 1:200.

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  IF Staining of human liver cancer using CL488-67300

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 CD107a antibody (CL488-67300, Clone: 3B2E9 ) at dilution of 1:200.

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  FC experiment of NK92 using CL488-67300

  1X10^6 NK92 were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CD107a / LAMP1 (CL488-67300, Clone:3B2E9) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: human liver cancer tissue
• Positive FC detected in: NK92

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

CL488-67300 targets CD107a / LAMP1 in IF, FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD107a / LAMP1 fusion protein Ag28869
• Full Name: lysosomal-associated membrane protein 1
• Calculated molecular weight: 417 aa, 45 kDa
• Observed molecular weight: 90-120 kDa
• GenBank accession number: BC021288
• Gene symbol: LAMP1
• Gene ID (NCBI): 3916
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.