CoraLite®532-conjugated CD107a / LAMP1 Monoclonal antibody

• 

  IF Staining of mouse liver using CL532-67300

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CoraLite®532 CD107a / LAMP1 antibody (CL532-67300, Clone: 3B2E9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using CL532-67300

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CoraLite®532 CD107a / LAMP1 antibody (CL532-67300, Clone: 3B2E9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD107a / LAMP1 Monoclonal Antibody for IF-P

Cat No. CL532-67300

Clone No.3B2E9

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF-P

Synonyms

CD107a, LAMP1, LAMP-1, LAMP 1, CD107 antigen-like family member A

---

Tested Applications

• Positive IF-P detected in: mouse liver tissue

This antibody is not recommended for immunocytofluorescent assays.

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL532-67300 targets CD107a / LAMP1 in IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28869

  Product name: Recombinant human CD107a protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 35-250 aa of BC021288

  Sequence: NGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNT

• Full Name: lysosomal-associated membrane protein 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 90-120 kDa
• GenBank Accession Number: BC021288
• Gene Symbol: LAMP1
• Gene ID (NCBI): 3916
• ENSEMBL Gene ID: ENSG00000185896
• Conjugate: CoraLite®532 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 537 nm / 560 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11279
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.