CD11B/Integrin alpha M Antibody

CD11B/Integrin alpha M Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1C7C2

Cat no : 66519-1-Ig

Synonyms

CD11B, CR 3 alpha chain, CR3A, Integrin alpha M, ITGAM, MAC 1, MAC1A, MO1A, Neutrophil adherence receptor, SLEB6



Tested Applications

Positive WB detected inTHP-1 cells, human bone marrow tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Positive FC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66519-1-Ig targets CD11B/Integrin alpha M in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Calculated molecular weight 93 kDa
Observed molecular weight 160 kDa
GenBank accession number NM_000632
Gene symbol ITGAM
Gene ID (NCBI) 3684
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ITGAM, also named as CD11B and CR3A, belongs to the integrin alpha chain family. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. ITGAM is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. It is also a receptor for fibrinogen, factor X and ICAM1. ITGAM recognizes P1 and P2 peptides of fibrinogen gamma chain.

Protocols

Product Specific Protocols
WB protocol for CD11B/Integrin alpha M antibody 66519-1-IgDownload protocol
IHC protocol for CD11B/Integrin alpha M antibody 66519-1-IgDownload protocol
IF protocol for CD11B/Integrin alpha M antibody 66519-1-IgDownload protocol
FC protocol for CD11B/Integrin alpha M antibody 66519-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

MBio

The Extracellular Domain of the β2 Integrin β Subunit (CD18) Is Sufficient for Escherichia coli Hemolysin and Aggregatibacter actinomycetemcomitans Leukotoxin Cytotoxic Activity.

Authors - Laura C Ristow
ratIF

Mol Ther Methods Clin Dev

Reversal of Hypoxic Pulmonary Hypertension by Hypoxia-Inducible Overexpression of Angiotensin-(1-7) in Pulmonary Endothelial Cells.

Authors - Man-Ling Liu
mouseIF

Kaohsiung J Med Sci

PMID: 32627922

Authors - Xue-Yue Zhou
ratWB

Food Funct

The profiling of bioactives in Akebia trifoliata pericarp and metabolites, bioavailability and in vivo anti-inflammatory activities in DSS-induced colitis mice.

Authors - Xiaoya Wang
mouseIF

J Exp Med

TIPE2 specifies the functional polarization of myeloid-derived suppressor cells during tumorigenesis.

Authors - Dehong Yan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Santhosh (Verified Customer) (04-15-2019)

Human FFPE tonsil tissue, HIER pH6

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
CD11B/Integrin alpha M Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:66519-1-Ig)