CD11B/Integrin Alpha M Monoclonal antibody

CD11B/Integrin Alpha M Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1C7C2

Cat no : 66519-1-Ig

Synonyms

CD11B, CD11B/Integrin Alpha M, CR 3 alpha chain, CR3A, Integrin alpha M, ITGAM, MAC 1, MAC1A, MO1A, Neutrophil adherence receptor, SLEB6



Tested Applications

Positive WB detected inTHP-1 cells, TF-1 cells, human bone marrow tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Positive FC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66519-1-Ig targets CD11B/Integrin Alpha M in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Calculated molecular weight 93 kDa
Observed molecular weight 160 kDa
GenBank accession numberNM_000632
Gene symbol ITGAM
Gene ID (NCBI) 3684
RRIDAB_2881882
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Protocols

Product Specific Protocols
WB protocol for CD11B/Integrin Alpha M antibody 66519-1-IgDownload protocol
IHC protocol for CD11B/Integrin Alpha M antibody 66519-1-IgDownload protocol
IF protocol for CD11B/Integrin Alpha M antibody 66519-1-IgDownload protocol
FC protocol for CD11B/Integrin Alpha M antibody 66519-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

J Exp Med

TIPE2 specifies the functional polarization of myeloid-derived suppressor cells during tumorigenesis.

Authors - Dehong Yan
FC

Bioact Mater

Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.

Authors - Haoran Wang
IF

Apoptosis

ITGAM-macrophage modulation as a potential strategy for treating neutrophilic Asthma: insights from bioinformatics analysis and in vivo experiments

Authors - Qian Yan
WB

J Neuroinflammation

C1q/TNF-related protein 4 restores leptin sensitivity by downregulating NF-κB signaling and microglial activation.

Authors - Liu Ye
IF

Front Immunol

Matrine Inhibits CNS Autoimmunity Through an IFN-β-Dependent Mechanism.

Authors - Yao-Juan Chu
humanIHC,IF

Front Immunol

Redox Regulator GLRX Is Associated With Tumor Immunity in Glioma.

Authors - Yuanhao Chang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Santhosh (Verified Customer) (04-15-2019)

Human FFPE tonsil tissue, HIER pH6

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
CD11B/Integrin Alpha M Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:66519-1-Ig)