|Positive WB detected in||THP-1 cells, human bone marrow tissue|
|Positive IHC detected in||human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue|
|Positive FC detected in||THP-1 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66519-1-Ig targets CD11B/Integrin Alpha M in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.
|Host / Isotype||Mouse / IgG2b|
|Full Name||integrin, alpha M (complement component 3 receptor 3 subunit)|
|Calculated molecular weight||93 kDa|
|Observed molecular weight||160 kDa|
|GenBank accession number||NM_000632|
|Gene ID (NCBI)||3684|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ITGAM, also named as CD11B and CR3A, belongs to the integrin alpha chain family. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. ITGAM is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. It is also a receptor for fibrinogen, factor X and ICAM1. ITGAM recognizes P1 and P2 peptides of fibrinogen gamma chain.
|Product Specific Protocols|
|WB protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig||Download protocol|
|IHC protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig||Download protocol|
|IF protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig||Download protocol|
|FC protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Santhosh (Verified Customer) (04-15-2019)
Human FFPE tonsil tissue, HIER pH6