|Positive WB detected in||THP-1 cells, TF-1 cells, human bone marrow tissue|
|Positive IHC detected in||human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue|
|Positive FC detected in||THP-1 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66519-1-Ig targets CD11B/Integrin Alpha M in WB, IHC, IF, FC,ELISA applications and shows reactivity with Human samples.
|Host / Isotype||Mouse / IgG2b|
|Full Name||integrin, alpha M (complement component 3 receptor 3 subunit)|
|Calculated molecular weight||93 kDa|
|Observed molecular weight||160 kDa|
|GenBank accession number||NM_000632|
|Gene ID (NCBI)||3684|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
|Product Specific Protocols|
|WB protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig||Download protocol|
|IHC protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig||Download protocol|
|IF protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig||Download protocol|
|FC protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Santhosh (Verified Customer) (04-15-2019)
Human FFPE tonsil tissue, HIER pH6