CD11B/Integrin Alpha M Monoclonal antibody
CD11B/Integrin Alpha M Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Cat no : 66519-1-Ig
Validation Data Gallery
|Positive WB detected in
|THP-1 cells, TF-1 cells, human bone marrow tissue
|Positive IHC detected in
|human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|human tonsillitis tissue
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:4000
|IHC : 1:500-1:2000
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 1:10-1:100
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66519-1-Ig targets CD11B/Integrin Alpha M in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.
|Host / Isotype
|Mouse / IgG2b
|integrin, alpha M (complement component 3 receptor 3 subunit)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
|Product Specific Protocols
|WB protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig
|IHC protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig
|IF protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig
|FC protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig
|Click here to view our Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Santhosh (Verified Customer) (04-15-2019)
Human FFPE tonsil tissue, HIER pH6