CD133 Monoclonal antibody
CD133 Monoclonal Antibody for FC, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (3)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
2B8A2
Cat no : 66666-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HT-29 cells, Caco-2 cells |
Positive IHC detected in | human kidney tissue, human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | HT-29 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 23 publications below |
IHC | See 10 publications below |
IF | See 11 publications below |
ELISA | See 1 publications below |
Product Information
66666-1-Ig targets CD133 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD133 fusion protein Ag13327 |
Full Name | prominin 1 |
Calculated molecular weight | 97 kDa |
Observed molecular weight | 115 kDa, 80-90 kDa |
GenBank accession number | BC012089 |
Gene symbol | PROM1 |
Gene ID (NCBI) | 8842 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors. CD133 is a highly glycosylated protein with an apparent molecular weight of 115-120 kDa. After the treatment of the lysates with glycosidase, CD133 shifted to a protein with an apparent molecular weight of 80-90 kDa (PMID: 23150174; 20068153).
Protocols
Product Specific Protocols | |
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WB protocol for CD133 antibody 66666-1-Ig | Download protocol |
IHC protocol for CD133 antibody 66666-1-Ig | Download protocol |
FC protocol for CD133 antibody 66666-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Int J Mol Sci Promoter Methylation of Cancer Stem Cell Surface Markers as an Epigenetic Biomarker for Prognosis of Oral Squamous Cell Carcinoma | ||
Pharmacol Res Sinomenine hydrochloride suppresses the stemness of breast cancer stem cells by inhibiting Wnt signaling pathway through down-regulation of WNT10B. | ||
Cell Physiol Biochem Gab2 Ablation Reverses the Stemness of HER2-Overexpressing Breast Cancer Cells. | ||
Front Cell Dev Biol Cyclic Stretch Induces Vascular Smooth Muscle Cells to Secrete Connective Tissue Growth Factor and Promote Endothelial Progenitor Cell Differentiation and Angiogenesis. | ||
Front Cell Dev Biol ENC1 Facilitates Colorectal Carcinoma Tumorigenesis and Metastasis via JAK2/STAT5/AKT Axis-Mediated Epithelial Mesenchymal Transition and Stemness. | ||
Front Cell Dev Biol m6A Modification-Mediated DUXAP8 Regulation of Malignant Phenotype and Chemotherapy Resistance of Hepatocellular Carcinoma Through miR-584-5p/MAPK1/ERK Pathway Axis. |