Validation Data Gallery
|Positive WB detected in||HT-29 cells, Caco-2 cells|
|Positive IHC detected in||human kidney tissue, human breast cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HT-29 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
66666-1-Ig targets CD133 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||CD133 fusion protein Ag13327|
|Full Name||prominin 1|
|Calculated molecular weight||97 kDa|
|Observed molecular weight||115 kDa, 80-90 kDa|
|GenBank accession number||BC012089|
|Gene ID (NCBI)||8842|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors. CD133 is a highly glycosylated protein with an apparent molecular weight of 115-120 kDa. After the treatment of the lysates with glycosidase, CD133 shifted to a protein with an apparent molecular weight of 80-90 kDa (PMID: 23150174; 20068153).
Mol Ther Oncolytics
ROS/KRAS/AMPK Signaling Contributes to Gemcitabine-Induced Stem-like Cell Properties in Pancreatic Cancer.
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The critical role of peroxiredoxin-2 in colon cancer stem cells.
Sinomenine hydrochloride suppresses the stemness of breast cancer stem cells by inhibiting Wnt signaling pathway through down-regulation of WNT10B.
Int J Mol Sci
Promoter Methylation of Cancer Stem Cell Surface Markers as an Epigenetic Biomarker for Prognosis of Oral Squamous Cell Carcinoma
Cell Physiol Biochem
Gab2 Ablation Reverses the Stemness of HER2-Overexpressing Breast Cancer Cells.
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Cyclic Stretch Induces Vascular Smooth Muscle Cells to Secrete Connective Tissue Growth Factor and Promote Endothelial Progenitor Cell Differentiation and Angiogenesis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Udesh (Verified Customer) (08-16-2023)
Worked well in WB at 1:1000