CD133 Monoclonal antibody

CD133 Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (3)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

39

CloneNo.

2B8A2

Cat no : 66666-1-Ig

Synonyms

AC133, Antigen AC133, CD133, CORD12, MCDR2, MSTP061, PROM1, PROM1 1, PROM1 1,2,3, PROM1 1,2,3,5,7, PROM1 1,6,7, PROM1 2, PROM1 2,3,4,5, prominin 1, Prominin like protein 1, PROML1, RP41, STGD4

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Validation Data Gallery

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  WB analysis of HT-29 using 66666-1-Ig

  Untreated and PNGase F-treated lysates of HT-29 cells and Caco-2 cells were subjected to SDS PAGE followed by western blot with 66666-1-Ig (CD133 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

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  IHC staining of human kidney using 66666-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66666-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66666-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:2100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66666-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:2100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66666-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66666-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66666-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:2100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66666-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:2100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HT-29 using 66666-1-Ig

  1X10^6 HT-29 cells were stained with 0.20 ug Anti-Human CD133 (66666-1-Ig, Clone:2B8A2) (red) or 0.20 ug isotype control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Cells were fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: HT-29 cells, Caco-2 cells
• Positive IHC detected in: human kidney tissue, human breast cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 23 publications below
• IHC: See 10 publications below
• IF: See 11 publications below
• ELISA: See 1 publications below

Product Information

66666-1-Ig targets CD133 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD133 fusion protein Ag13327
• Full Name: prominin 1
• Calculated molecular weight: 97 kDa
• Observed molecular weight: 115 kDa, 80-90 kDa
• GenBank accession number: BC012089
• Gene symbol: PROM1
• Gene ID (NCBI): 8842
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors. CD133 is a highly glycosylated protein with an apparent molecular weight of 115-120 kDa. After the treatment of the lysates with glycosidase, CD133 shifted to a protein with an apparent molecular weight of 80-90 kDa (PMID: 23150174; 20068153).

Publications

Species	Application	Title
human	IHC	Int J Mol Sci; Promoter Methylation of Cancer Stem Cell Surface Markers as an Epigenetic Biomarker for Prognosis of Oral Squamous Cell Carcinoma; Authors - Yu Kyeong Han
human	IF	Pharmacol Res; Sinomenine hydrochloride suppresses the stemness of breast cancer stem cells by inhibiting Wnt signaling pathway through down-regulation of WNT10B.; Authors - Xiaomei Li
human	WB,IHC	Cell Physiol Biochem; Gab2 Ablation Reverses the Stemness of HER2-Overexpressing Breast Cancer Cells.; Authors - Peng Zhang
rat	IHC,IF	Front Cell Dev Biol; Cyclic Stretch Induces Vascular Smooth Muscle Cells to Secrete Connective Tissue Growth Factor and Promote Endothelial Progenitor Cell Differentiation and Angiogenesis.; Authors - Jing Yan
human	WB	Front Cell Dev Biol; ENC1 Facilitates Colorectal Carcinoma Tumorigenesis and Metastasis via JAK2/STAT5/AKT Axis-Mediated Epithelial Mesenchymal Transition and Stemness.; Authors - Ying Cui
human	WB	Front Cell Dev Biol; m6A Modification-Mediated DUXAP8 Regulation of Malignant Phenotype and Chemotherapy Resistance of Hepatocellular Carcinoma Through miR-584-5p/MAPK1/ERK Pathway Axis.; Authors - Zefeng Liu