CD133 Monoclonal antibody

CD133 Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (3)

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

CloneNo.

2B8A2

Cat no : 66666-1-Ig

Synonyms

AC133, Antigen AC133, CD133, CORD12, MCDR2, MSTP061, PROM1, PROM1 1, PROM1 1,2,3, PROM1 1,2,3,5,7, PROM1 1,6,7, PROM1 2, PROM1 2,3,4,5, prominin 1, Prominin like protein 1, PROML1, RP41, STGD4



Tested Applications

Positive WB detected inHT-29 cells, Caco-2 cells
Positive IHC detected inhuman kidney tissue, human breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

66666-1-Ig targets CD133 in WB, IHC, IF, FC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD133 fusion protein Ag13327
Full Name prominin 1
Calculated molecular weight 97 kDa
Observed molecular weight 115 kDa, 80-90 kDa
GenBank accession numberBC012089
Gene symbol PROM1
Gene ID (NCBI) 8842
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors. CD133 is a highly glycosylated protein with an apparent molecular weight of 115-120 kDa. After the treatment of the lysates with glycosidase, CD133 shifted to a protein with an apparent molecular weight of 80-90 kDa (PMID: 23150174; 20068153).

Protocols

Product Specific Protocols
WB protocol for CD133 antibody 66666-1-IgDownload protocol
IHC protocol for CD133 antibody 66666-1-IgDownload protocol
FC protocol for CD133 antibody 66666-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Int J Oncol

Sphere‑forming assay vs. organoid culture: Determining long‑term stemness and the chemoresistant capacity of primary colorectal cancer cells.

Authors - Hui Zhao
humanIHC

Mol Ther Oncolytics

ROS/KRAS/AMPK Signaling Contributes to Gemcitabine-Induced Stem-like Cell Properties in Pancreatic Cancer.

Authors - Hengqiang Zhao
humanWB

Med Sci Monit

RNA Binding Protein RNPC1 Suppresses the Stemness of Human Endometrial Cancer Cells via Stabilizing MST1/2 mRNA.

Authors - XingMei Wu
humanIF

Mol Med Rep

lncRNA TPTEP1 inhibits stemness and radioresistance of glioma through miR‑106a‑5p‑mediated P38 MAPK signaling.

Authors - Ting Tang
humanWB

Oncol Lett

Benzidine promotes the stemness of bladder cancer stem cells via activation of the Sonic hedgehog pathway.

Authors - Dengdian Wang
humanWB

Environ Toxicol

Ginsenoside Rg3 attenuates the osimertinib resistance by reducing the stemness of non-small cell lung cancer cells.

Authors - Qinquan Tan