CoraLite®594-conjugated CD146/MCAM Monoclonal antibody

CD146/MCAM Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4D8A9

Cat no : CL594-66153

Synonyms

CD146, MCAM, MUC18



Tested Applications

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66153-1-Ig

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL594-66153 is CD146/MCAM Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD146/MCAM fusion protein Ag11855
Full Name melanoma cell adhesion molecule
Calculated molecular weight 646 aa, 72 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC056418
Gene symbol MCAM
Gene ID (NCBI) 4162
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band.

Protocols

Product Specific Protocols
FC protocol for CL594 CD146/MCAM antibody CL594-66153Download protocol
Standard Protocols
Click here to view our Standard Protocols