Anti-Human CD158Z/KIR3DL3 Rabbit Recombinant Antibody

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  FC experiment of Transfected CHO-K1 using 98628-1-RR

  1x10^6 Transfected CHO-K1 or Transfected CD158z or Transfected CD158g were surface stained with 0.25 ug Anti-Human CD158Z/KIR3DL3 Rabbit RecAb (98628-1-RR, Clone:250254F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

• 

  FC experiment of Transfected CHO-K1 using 98628-1-RR

  1x10^6 Transfected CHO-K1 or Transfected CD158z or Transfected CD158g were surface stained with 0.25 ug Anti-Human CD158Z/KIR3DL3 Rabbit RecAb (98628-1-RR, Clone:250254F12) (red), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

CD158Z/KIR3DL3 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98628-1-RR

Clone No.250254F12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

KIR3DL3, CD158Z, KIR3DL7, KIR44, KIRC1

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Tested Applications

• Positive FC detected in: Transfected CHO-K1 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98628-1-RR targets CD158Z/KIR3DL3 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg4358

  Product name: Recombinant Human KIR3DL3 protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 26-322 aa of /

  Sequence: QDKPFLSAWPGTVVSEGQHVTLQCRSRLGFNEFSLSKEDGMPVPELYNRIFRNSFLMGPVTPAHAGTYRCCSSHPHSPTGWSAPSNPVVIMVTGVHRKPSLLAHPGPLVKSGETVILQCWSDVRFERFLLHREGITEDPLRLVGQLHDAGSQVNYSMGPMTPALAGTYRCFGSVTHLPYELSAPSDPLDIVVVGLYGKPSLSAQPGPTVQAGENVTLSCSSRSLFDIYHLSREAEAGELRLTAVLRVNGTFQANFPLGPVTHGGNYRCFGSFRALPHAWSDPSDPLPVSVTGNSRHL

• Full Name: killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 3
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: /
• Gene Symbol: KIR3DL3
• Gene ID (NCBI): 115653
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N743
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD158z, also known as KIR3DL3, is an inhibitory receptor belonging to the Killer-cell Immunoglobulin-like Receptor (KIR) family. It plays a critical role in the regulation of the human immune system, specifically by modulating the activity of natural killer (NK) cells and certain T cells