CD1A Monoclonal antibody, PBS Only

• 

  WB analysis of pig thymus using 60173-3-Ig (same clone as 60173-3-PBS)

  pig thymus tissue were subjected to SDS PAGE followed by western blot with 60173-3-Ig (CD1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60173-3-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 60173-3-Ig (same clone as 60173-3-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60173-3-Ig (CD1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60173-3-PBS in a different storage buffer formulation.

CD1A Monoclonal Antibody for WB, Indirect ELISA

Cat No. 60173-3-PBS

Clone No.1H7B2

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, Indirect ELISA

Synonyms

1H7B2, CD1, CD1a molecule, FCB6, HTA1

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Product Information

60173-3-PBS targets CD1A in WB, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11304

  Product name: Recombinant human CD1A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-284 aa of BC031645

  Sequence: MWNWLKEPLSFHVIWIASFYNHSWKQNLVSGWLSDLQTHTWDSNSSTIVFLWPWSRGNFSNEEWKELETLFRIRTIRSFEGIRRYAHELQFEYPFEIQVTGGCELHSGKVSGSFLQLAYQGSDFVSFQNNSWLPYPVAGNMAKHFCKVLNQNQHENDITHNLLSDTCPRFILGLLDAGKAHLQRQVKPEAWLSHGPSPGPGHLQLVCHVSGFYPKPVWVMWMRGEQEQQGTQRGDILPSADGTWYLRATLEVAAGEAADLSCRVKHSSLEGQDIVLYWEHHSSV

• Full Name: CD1a molecule
• Calculated Molecular Weight: 327 aa, 37 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC031645
• Gene Symbol: CD1A
• Gene ID (NCBI): 909
• RRID: AB_2881353
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P06126
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD1a is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. During protein synthesis and maturation, CD1 family members bind endogenous lipids that are replaced by lipid or glycolipid antigens when the proteins are internalized and pass through endosomes, before trafficking back to the cell surface.