Filter: AllWBIHCIF-P

WB analysis using 81525-1-RR (same clone as 81525-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 81525-1-RR (CD206 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 81525-1-RR (same clone as 81525-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 81525-1-RR (same clone as 81525-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 81525-1-RR (same clone as 81525-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 81525-1-RR (same clone as 81525-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 81525-1-RR (same clone as 81525-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 81525-1-RR (same clone as 81525-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 81525-1-RR (same clone as 81525-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 81525-1-RR (same clone as 81525-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 81525-1-RR (same clone as 81525-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 81525-1-RR (same clone as 81525-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 81525-1-RR (same clone as 81525-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 81525-1-RR (same clone as 81525-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 81525-1-RR (same clone as 81525-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 81525-1-RR (same clone as 81525-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.
IF Staining of human placenta using 81525-1-RR (same clone as 81525-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD206 antibody (81525-1-RR, Clone: 1G15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.
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Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD206 antibody (81525-1-RR, Clone: 1G15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.
IF Staining of human placenta using 81525-1-RR (same clone as 81525-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD206 antibody (81525-1-RR, Clone: 1G15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD206 antibody (81525-1-RR, Clone: 1G15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

CD206 Recombinant monoclonal antibody, PBS Only

CD206 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 81525-1-PBS

Clone No.1G15

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

1G15, CLEC13D, CLEC13DL, C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

81525-1-PBS targets CD206 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity	human, rat, pig
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	fusion protein Predict reactive species
Full Name	mannose receptor, C type 1
Calculated Molecular Weight	166 kDa
Observed Molecular Weight	170 kDa
GenBank Accession Number	NM_002438
Gene Symbol	CD206
Gene ID (NCBI)	4360
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P22897
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.