Tested Applications
| Positive WB detected in | pig liver tissue, rat liver tissue, human placenta tissue |
| Positive IHC detected in | human lung cancer tissue, human colon cancer tissue, human liver tissue, human lung tissue, human lymphoma tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human placenta tissue |
| Positive IF/ICC detected in | PMA, IL-4, and IL-13 treated THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
81525-1-RR targets CD206 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, rat, pig samples.
| Tested Reactivity | human, rat, pig |
| Cited Reactivity | human, rat, pig, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | mannose receptor, C type 1 |
| Calculated Molecular Weight | 166 kDa |
| Observed Molecular Weight | 170 kDa |
| GenBank Accession Number | NM_002438 |
| Gene Symbol | CD206 |
| Gene ID (NCBI) | 4360 |
| RRID | AB_2935558 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P22897 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD206 antibody 81525-1-RR | Download protocol |
| IHC protocol for CD206 antibody 81525-1-RR | Download protocol |
| WB protocol for CD206 antibody 81525-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD206 Recombinant Monoclonal Antibody (Cat# 81525-1-RR) has 39 citations. These appear in journals including Nature Communications, ACS Nano, Theranostics, Advanced Science, Cell Death Discovery, Journal of Immunotherapy of Cancer, Stem Cell Research & Therapy, International Journal of Immunopharmacology, Scientific Reports, and Cytokine. Research fields center on macrophage M2 polarization, neuroinflammation and ischemic stroke, tumor microenvironment remodeling, cancer immunotherapy, wound healing, and metabolic/inflammatory diseases.
| Species | Application | Title |
|---|---|---|
Exploration (Beijing) Specifically Breaking Through the Injured Blood-Brain Barrier With Tannic Acid-Based Nanomedicine for Ischemic Stroke Ischemia Reperfusion Treatment. | ||
Nat Commun Acid-resistant chemotactic DNA micromotors for probiotic delivery in inflammatory bowel disease | ||
ACS Nano Unlocking Neuroprotection: Simultaneous Suppression of Mitochondrial Energetic Collapse and Oxidative-Inflammatory Vortex for Ischemia-Reperfusion Brain Injury. | ||
Theranostics Hsa_circ_0058495-mediated IGF2BP2 ubiquitination and m6A modification of MEKK1 promote the progression of PDAC. | ||
Adv Sci (Weinh) Integrated Single-Cell and Spatial Analysis Reveals a Metabolic-Immune Axis Driving Aortic Dissection. | ||
Adv Sci (Weinh) Intrinsically Mitochondria-Targeting Nanozyme via Coordination-Assembly of Natural Quercetin for Cascade Antioxidant Therapy of Cerebral Ischemia-Reperfusion Injury. |
Reviews
What customers say
The single review for this antibody gives it a 5-star rating. The user reports it outperforms other CD206 antibodies they have tested, producing very robust immunofluorescent signals when used at a 1:500 dilution on mouse nerve meninges tissue. The application was immunofluorescence, and the antibody successfully labeled a subset of macrophages. Overall, the reviewer highly recommends this antibody for CD206 detection in mouse tissues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kenzo (Verified Customer) (08-15-2023) | I highly recommend this antibody as it performs best among CD206 antibodies that we have tested. I was able to detect very robust immunofluorescent signals with this antibody from a subset of macrophages in mouse nerve tissues.
|
