CD239/BCAM Recombinant monoclonal antibody

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  WB analysis using 87430-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87430-1-RR (CD239/BCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity of 87430-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87430-1-RR against Mouse CD239/BCAM were performed. The affinity constant is 4.73 nM.

CD239/BCAM Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87430-1-RR

Clone No.251702A10

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

Basal cell adhesion molecule, Bcam, B-CAM cell surface glycoprotein, CD239, Gplu

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Tested Applications

• Positive WB detected in: mouse lung tissue, rat lung tissue, mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87430-1-RR targets CD239/BCAM in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg6306

  Product name: Recombinant mouse CD239/BCAM protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 26-541 aa of NM_020486.2

  Sequence: ELHVSVPPRVEVMRGEQVALDCTPREHPEHYVLEWFLVDGTGARHRLASVEPQGSEFLGTVHSLGRVPPYEVDSRGRLVIAKVQVGDGRDYVCVVKAGAAGTSEATSSVRVFATPEDTEVSPNKGTLSVMDQFAQEIATCSSNNGNPVPRITWYRNGQRLEVPMEVNQKGYITIRTVREASGLYSLTSTLYLRLHKDDRDANFHCAAHYDLPSGQHGRLDSHTFRLTLHYPTEHVEFWVGSPSTTEGWVREGDAVQLLCQGDGSPSPEYSFFRQQGTQEEQLNVNLKGNLTLERVHRNQSGIYGCRVEDYDADEEVQLVKKLKLHVAYLDPLELSVPEELFVFLNSSSTVVNCSARGLPTPTVRWTKDSVTLADGPMLSLQSVTFDSAGTYTCEASTPTVPLLSRTQSFQLIVQGAPELKPNEIMPKSGNSWTEGDEVMLTCSARGFPEPKLTWSQRGDTPAEPPFEGRGWKSSSLMVKVTSALSREGVSCEASNIHGKKGHVFHFGSVAPQTAQA

• Full Name: basal cell adhesion molecule
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 67 kDa, 78-85 kDa
• GenBank Accession Number: NM_020486.2
• Gene Symbol: Bcam
• Gene ID (NCBI): 57278
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9R069
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Basal cell adhesion molecule (Lu/BCAM) is a membrane-bound glycoprotein of the immunoglobulin superfamily (IgSF), functioning as a receptor for the extracellular matrix protein, laminin. BCAM (Lutheran/basal cell-adhesion molecule, a glycoprotein) belongs to the immunoglobulin superfamily which contains both Lu blood group and BCAM tumor-associated antigens. Proteomics analysis suggests that BCAM might be a potential biomarker for pancreatic cancer (PMID: 19199705). BCAM, involved in cell adhesion and migration, can also promote tumor metastasis and has been elucidated to play a functional role in the metastasis of thyroid cancer and gastric cancer (PMID: 10728810, 35941663). De-glycosylated BCAM has a preidicted molecular weight of 67 kDa. Two BCAM glycoprotein (gp) isoforms are present on human RBCs, Lu (85 kDa) and Lu(v13) (78 kDa); they result from the alternative splicing of intron 13 and differ by the lengths of their cytoplasmic domains (PMID: 23160466).