Anti-Human CD28 (CD28.2)

CD28 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa


Human and More (1)


IHC, FC, Cell treatment





Cat no : 65099-1-Ig


CD28, CD28 molecule, Tp44

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

65099-1-Ig targets CD28 in IHC, FC, Cell treatment applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name CD28 molecule
Calculated molecular weight 220 aa, 25 kDa
GenBank accession numberBC093698
Gene symbol CD28
Gene ID (NCBI) 940
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).


Product Specific Protocols
FC protocol for CD28 antibody 65099-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cancer Cell Int

Comprehensive analysis of prognostic immune-related genes and drug sensitivity in cervical cancer.

Authors - Ya-Nan Pi

J Transl Med

Scoring model based on the signature of non-m6A-related neoantigen-coding lncRNAs assists in immune microenvironment analysis and TCR-neoantigen pair selection in gliomas

Authors - Wenbo Zhao
humanCell treatment

Aging (Albany NY)

An integrated bioinformatic investigation of focal adhesion-related genes in glioma followed by preliminary validation of COL1A2 in tumorigenesis

Authors - Guojun Yao