CD315/PTGFRN Recombinant monoclonal antibody

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  WB analysis of LN-229 using 87504-1-RR

  LN-229 cells were subjected to SDS PAGE followed by western blot with 87504-1-RR (CD315/PTGFRN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 87504-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87504-1-RR (CD315/PTGFRN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity of 87504-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87504-1-RR against Human CD315/PTGFRN were performed. The affinity constant is below 1 pM.

CD315/PTGFRN Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87504-1-RR

Clone No.252486D2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

PTGFRN, CD315, EWIF, FPRP, KIAA1436

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Tested Applications

• Positive WB detected in: U-251 cells, LN-229 cells, U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87504-1-RR targets CD315/PTGFRN in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5010

  Product name: Recombinant human PTGFRN protein (C-rFc)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 26-832 aa of BC152454

  Sequence: VPTATLVRVVGTELVIPCNVSDYDGPSEQNFDWSFSSLGSSFVELASTWEVGFPAQLYQERLQRGEILLRRTANDAVELHIKNVQPSDQGHYKCSTPSTDATVQGNYEDTVQVKVLADSLHVGPSARPPPSLSLREGEPFELRCTAASASPLHTHLALLWEVHRGPARRSVLALTHEGRFHPGLGYEQRYHSGDVRLDTVGSDAYRLSVSRALSADQGSYRCIVSEWIAEQGNWQEIQEKAVEVATVVIQPSVLRAAVPKNVSVAEGKELDLTCNITTDRADDVRPEVTWSFSRMPDSTLPGSRVLARLDRDSLVHSSPHVALSHVDARSYHLLVRDVSKENSGYYYCHVSLWAPGHNRSWHKVAEAVSSPAGVGVTWLEPDYQVYLNASKVPGFADDPTELACRVVDTKSGEANVRFTVSWYYRMNRRSDNVVTSELLAVMDGDWTLKYGERSKQRAQDGDFIFSKEHTDTFNFRIQRTTEEDRGNYYCVVSAWTKQRNNSWVKSKDVFSKPVNIFWALEDSVLVVKARQPKPFFAAGNTFEMTCKVSSKNIKSPRYSVLIMAEKPVGDLSSPNETKYIISLDQDSVVKLENWTDASRVDGVVLEKVQEDEFRYRMYQTQVSDAGLYRCMVTAWSPVRGSLWREAATSLSNPIEIDFQTSGPIFNASVHSDTPSVIRGDLIKLFCIITVEGAALDPDDMAFDVSWFAVHSFGLDKAPVLLSSLDRKGIVTTSRRDWKSDLSLERVSVLEFLLQVHGSEDQDFGNYYCSVTPWVKSPTGSWQKEAEIHSKPVFITVKMDVLNAFKYP

• Full Name: prostaglandin F2 receptor negative regulator
• Calculated Molecular Weight: 879 aa, 99 kDa
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: BC152454
• Gene Symbol: PTGFRN
• Gene ID (NCBI): 5738
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9P2B2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTGFRN (Prostaglandin F2 Receptor Negative Regulator), also known as CD315, is a trans-membrane protein that modulates prostaglandin F2α signaling and links the tetraspanin web to the cytoskeleton, it is now recognized as a driver of metastasis; high expression correlates with aggressive proliferation, migration, and spheroid growth in epidermoid carcinoma, medulloblastoma, and mesothelioma, while silencing PTGFRN impairs these traits and disrupts autophagy (PMID: 38924562). The calculated value of PTGFRN is 98 kDa. It has multiple glycosylation sites and its molecular weight after glycosylation is approximately 100 - 130 kDa.