Validation Data Gallery
|Positive WB detected in||Jurkat cells, human spleen tissue, pig spleen tissue, MOLT-4 cells, mouse thymus tissue, rat thymus tissue|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human tonsillitis tissue, human breast cancer tissue, mouse spleen tissue, mouse colon tissue, human pancreas cancer tissue, human lymphoma tissue, human lung cancer tissue, rat spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue|
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17617-1-AP targets CD3 in WB, IP, IHC, IF, FC, Cell treatment, ELISA applications and shows reactivity with human, pig, mouse, rat samples.
|Tested Reactivity||human, pig, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD3 fusion protein Ag11797|
|Full Name||CD3e molecule, epsilon (CD3-TCR complex)|
|Calculated molecular weight||207 aa, 23 kDa|
|Observed molecular weight||23-25 kDa|
|GenBank accession number||BC049847|
|Gene ID (NCBI)||916|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a polyclonal antibody directed against the epsilon chain of human CD3 molecule.
|Product Specific Protocols|
|WB protocol for CD3 antibody 17617-1-AP||Download protocol|
|IHC protocol for CD3 antibody 17617-1-AP||Download protocol|
|IF protocol for CD3 antibody 17617-1-AP||Download protocol|
|IP protocol for CD3 antibody 17617-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Emma (Verified Customer) (11-29-2021)
This works really well by IF on FFPE tissue. We used a 1:50 dilution and Tris-EDTA antigen retrieval.