CD3 Polyclonal antibody

CD3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig, mouse, rat

Applications

WB, IP, IHC, IF, FC, Cell treatment, ELISA

Conjugate

Unconjugated

Cat no : 17617-1-AP

Synonyms

CD3, CD3 epsilon, CD3E, CD3-epsilon, T3E, TCRE



Tested Applications

Positive WB detected inJurkat cells, human spleen tissue, pig spleen tissue, MOLT-4 cells, mouse thymus tissue, rat thymus tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue, mouse spleen tissue, human lung cancer tissue, human breast cancer tissue, mouse colon tissue, human lymphoma tissue, human pancreas cancer tissue, rat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17617-1-AP targets CD3 in WB, IP, IHC, IF, FC, Cell treatment, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Reactivity human, pig, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD3 fusion protein Ag11797
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Calculated molecular weight 207 aa, 23 kDa
Observed molecular weight 23-25 kDa
GenBank accession numberBC049847
Gene symbol CD3E
Gene ID (NCBI) 916
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a polyclonal antibody directed against the epsilon chain of human CD3 molecule.

Protocols

Product Specific Protocols
WB protocol for CD3 antibody 17617-1-APDownload protocol
IHC protocol for CD3 antibody 17617-1-APDownload protocol
IF protocol for CD3 antibody 17617-1-APDownload protocol
IP protocol for CD3 antibody 17617-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nat Commun

Engineered triple inhibitory receptor resistance improves anti-tumor CAR-T cell performance via CD56.

Authors - Fan Zou
humanIHC

Cancer Res

M6A Demethylase ALKBH5 Regulates PD-L1 Expression and Tumor Immunoenvironment in Intrahepatic Cholangiocarcinoma.

Authors - Xinyao Qiu
mouseIHC

Br J Pharmacol

Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A

Authors - Lu Zhang
mouseFC

Bioact Mater

Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.

Authors - Haoran Wang
humanIHC

Cell Mol Immunol

The innate immune effector ISG12a promotes cancer immunity by suppressing the canonical Wnt/β-catenin signaling pathway.

Authors - Rilin Deng
humanIHC,FC

Clin Transl Med

c-Myb facilitates immune escape of esophageal adenocarcinoma cells through the miR-145-5p/SPOP/PD-L1 axis.

Authors - Lan Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Emma (Verified Customer) (11-29-2021)

This works really well by IF on FFPE tissue. We used a 1:50 dilution and Tris-EDTA antigen retrieval.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Prostate
CD3 Antibody Immunofluorescence validation (1:50 dilution) in Prostate (Cat no:17617-1-AP)