Tested Applications
| Positive WB detected in | Raji cells, Daudi cells, Ramos cells |
| Positive IHC detected in | human tonsillitis tissue, human colon tissue, human tonsillitis tissue, human appendicitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Positive IF/ICC detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:15000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60271-1-Ig targets CD20 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17309 Product name: Recombinant human MS4A1,CD20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 141-297 aa of BC002807 Sequence: IKISHFLKMESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIFAFFQELVIAGIVENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSPIENDSSP Predict reactive species |
| Full Name | membrane-spanning 4-domains, subfamily A, member 1 |
| Calculated Molecular Weight | 297 aa, 33 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC002807 |
| Gene Symbol | CD20 |
| Gene ID (NCBI) | 931 |
| RRID | AB_2881391 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P11836 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD20 antibody 60271-1-Ig | Download protocol |
| IHC protocol for CD20 antibody 60271-1-Ig | Download protocol |
| WB protocol for CD20 antibody 60271-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD20 Monoclonal antibody (Cat# 60271-1-Ig) has 52 citations spanning journals including Cancer Cell, Nature Communications, Hepatology, Science Translational Medicine, Theranostics, Advanced Science, Cell Death & Disease, Cancer Letters, Journal for Immunotherapy of Cancer, Clinical Cancer Research, Arthritis & Rheumatology, FASEB Journal, and Frontiers in Immunology, among others. Research fields encompass tumor immunology, tertiary lymphoid structures, B-cell biology, immune microenvironment characterization, immunotherapy response, liver fibrosis, autoimmune diseases, and xenotransplantation.
| Species | Application | Title |
|---|---|---|
Cancer Cell Remodeling of the immune and stromal cell compartment by PD-1 blockade in mismatch repair-deficient colorectal cancer | ||
Nat Commun Lymph nodes molecular subtypes unravel lymph nodes heterogeneity and clinical implications in colorectal cancer | ||
Nat Commun Neoadjuvant with low-dose radiotherapy, tislelizumab, albumin-bound paclitaxel, and cisplatin for resectable locally advanced head and neck squamous cell carcinoma: phase II single-arm trial | ||
Hepatology Hepatocyte-derived MASP1-enriched small extracellular vesicles activate HSCs to promote liver fibrosis | ||
Int J Oral Sci Targeting IFN-I driven tertiary lymphoid structures halts B cell-mediated bone loss in periodontitis. | ||
Sci Transl Med The m6A demethylase FTO links TLR7 to mitochondrial oxidation driving age-associated B cell formation in systemic lupus erythematosus |
