Anti-Mouse CD4 (RM4-4) Rat IgG2a Recombinant Antibody

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  FC experiment of mouse splenocytes using 65655-1-TR

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD4 (RM4-4) Rat IgG2a RecAb (65655-1-TR, Clone: RM4-4) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3), and FITC anti-Rat IgG2a antibody. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD8a (53-6.7) (CL647-65069, Clone: 53-6.7). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 65655-1-TR

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD4 (RM4-4) Rat IgG2a RecAb (65655-1-TR, Clone:RM4-4) (red) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) (blue), and FITC anti-Rat IgG2a antibody. Cells were not fixed.

CD4 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 65655-1-TR

Clone No.RM4-4

Host / Isotype

Rat / IgG2a

Reactivity

mouse

Applications

FC

Synonyms

CD4 antigen, L3T4, Ly 4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65655-1-TR targets CD4 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: CD4 antigen
• GenBank Accession Number: BC039137
• Gene Symbol: Cd4
• Gene ID (NCBI): 12504
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).