Anti-Mouse CD4 (RM4-4) Rat IgG2a Recombinant Antibody, PBS Only

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  FC experiment of mouse splenocytes using 65655-1-TR (same clone as 65655-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD4 (RM4-4) Rat IgG2a RecAb (65655-1-TR, Clone: RM4-4) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3), and FITC anti-Rat IgG2a antibody. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD8a (53-6.7) (CL647-65069, Clone: 53-6.7). Cells were not fixed. This data was developed using the same antibody clone with 65655-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 65655-1-TR (same clone as 65655-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD4 (RM4-4) Rat IgG2a RecAb (65655-1-TR, Clone:RM4-4) (red) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) (blue), and FITC anti-Rat IgG2a antibody. Cells were not fixed. This data was developed using the same antibody clone with 65655-1-PBS in a different storage buffer formulation.

CD4 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 65655-1-PBS

Clone No.RM4-4

Host / Isotype

Rat / IgG2a

Reactivity

mouse

Applications

FC

Synonyms

CD4 antigen, L3T4, Ly 4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3

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Product Information

65655-1-PBS targets CD4 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: CD4 antigen
• GenBank Accession Number: BC039137
• Gene Symbol: Cd4
• Gene ID (NCBI): 12504
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).