Tested Applications
| Positive FC detected in |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65209-1-Ig targets IgG2a Isotype Control in FC applications and shows reactivity with n/a samples.
| Tested Reactivity | n/a |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | IgG2a Isotype Control |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2918471 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
The 2A3 immunoglobulin is useful as an isotype-matched control. The 2A3 immunolglobulin binds to TNP (trinitrophenol), a hapten not expressed on mouse or human cells. It is used as an isotype control for rat IgG2a antibodies. This antibody has been quality-tested for flow cytometry as negative control.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IgG2a Isotype Control antibody 65209-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Rat IgG2a Isotype Control (2A3) (Cat# 65209-1-Ig) has a total of 3 citations. One published in Nature Communications (IF 18.1, 2026) investigated cardiomyocyte CDK9-mediated NF-κB phosphorylation driving cardiac inflammation and remodeling, using this antibody in flow cytometry as a negative control. The research field encompasses cardiovascular biology and inflammatory signaling.
| Species | Application | Title |
|---|---|---|
Nat Commun Cardiomyocyte Cyclin-dependent kinase 9 directly binds to and phosphorylates NF-κB p65 subunit to drive cardiac inflammation and remodeling. |
