PE Anti-Human CD4 (OKT4)

CD4 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

Human and More (1)

Applications

FC, FC (Intra)

Conjugate

PE Fluorescent Dye

Publications

1

CloneNo.

OKT4

Cat no : PE-65134

Synonyms

CD4, CD4 molecule, CD4mut

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Validation Data Gallery

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  FC experiment of human PBMCs using PE-65134

  1x10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD4 (OKT4) (PE-65134, Clone: OKT4) or PE Mouse IgG2b Isotype Control (MPC-11) (PE-65128, Clone: MPC-11), and 5 ul CoraLite® Plus 647 Anti-Human CD3 (UCHT1) (CL647-65151, Clone: UCHT1). Cells were not fixed.

• 

  FC experiment of human PBMCs using PE-65134

  1x10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD4 (OKT4) (PE-65134, Clone:OKT4) or PE Mouse IgG2b Isotype Control (MPC-11) (PE-65128, Clone: MPC-11). Cells were not fixed.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65134 targets CD4 in FC, FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human peripheral T cells
• Full Name: CD4 molecule
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: BC025782
• Gene Symbol: CD4
• Gene ID (NCBI): 920
• RRID: AB_2883917
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Publications

Species	Application	Title
human	FC (Intra)	Cardiovasc Res; Senescent CD8+ T cells: a novel risk factor in atrial fibrillation; Authors - Xiang Li