Tested Applications
| Positive WB detected in | J774A.1 cells, RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31446-1-AP targets CD40 in WB, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein Predict reactive species |
| Full Name | CD40 antigen |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | NM_011611.2 |
| Gene Symbol | Cd40 |
| Gene ID (NCBI) | 21939 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P27512-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD40, also known as TNFRSF5 or Bp50, is a member of the tumor necrosis factor (TNF) receptor (TNFR) family. It is expressed on a wide range of cell types including B cells, macrophages, and dendritic cells (PMID: 19426221). CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD40 antibody 31446-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
