|Positive WB detected in||Jurkat cells, human peripheral blood platelets|
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Sample-dependent, check data in validation data gallery|
66502-1-Ig targets CD40L/CD154 in WB, IHC, FC, ELISA applications and shows reactivity with Human, rat samples.
|Tested Reactivity||Human, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||CD40L/CD154 fusion protein Ag24941|
|Full Name||CD40 ligand|
|Calculated molecular weight||261 aa, 29 kDa|
|Observed molecular weight||30 kDa|
|GenBank accession number||BC071754|
|Gene ID (NCBI)||959|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.
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