Anti-Human CD45 Rabbit Recombinant Antibody

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  IF Staining of Jurkat using 98117-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45 antibody (98117-1-RR, Clone: 241670E10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of human peripheral blood leukocytes using 98117-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD45 Rabbit Recombinant Antibody (98117-1-RR, Clone: 241670E10) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98117-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98117-1-RR against Human CD45 were performed. The affinity constant is 2.58 nM.

CD45 Uni-rAb^® Recombinant Antibody for IF/ICC, FC

Cat No. 98117-1-RR

Clone No.241670E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC

Synonyms

EC:3.1.3.48, GP180, L CA, LCA, L-CA

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Tested Applications

• Positive IF/ICC detected in: Jurkat cells
• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98117-1-RR targets CD45 in IF/ICC, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2486

  Product name: recombinant human CD45(220-577) protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 10007019 of

  Sequence:

• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 147 kDa
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• ENSEMBL Gene ID: ENSG00000081237
• RRID: AB_3672264
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P08575
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).