Mouse CD45 Recombinant monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA sample test of MP00412-4

  The mean mouse CD45 concentration was determined to be 39.3 ng/mL in RAW 264.7 cell extract based on a 4.8 mg/mL extract load.

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  Sandwich ELISA sample test of MP00412-4

  RAW 264.7 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for mouse CD45, and measured 154.9 pg/mL.

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  Sandwich ELISA standard curve of MP00412-4

  Sandwich ELISA standard curve of MP00412-4, Mouse CD45 Recombinant Matched Antibody Pair - PBS only. 83396-14-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0845. 83396-7-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL.

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  Affinity and Kinetic Characterization of 83396-7-PBS

  Biolayer interferometry (BLl) kinetic assays of 83396-7-PBS against Mouse CD45 were performed. The affinity constant is 0.595 nM.

CD45 Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Cat No. 83396-7-PBS

Clone No.240920A8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Sandwich ELISA, Indirect ELISA

Synonyms

Ptprc, EC:3.1.3.48, LCA, L-CA, Leukocyte common antigen

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Product Information

83396-7-PBS targets CD45 as part of a matched antibody pair:

MP00412-4: 83396-14-PBS capture and 83396-7-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0845

  Product name: Recombinant Mouse CD45 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 26-566 aa of NM_001111316.2

  Sequence: QTPTPSDELSTTENALLLPQSDPLPARTTESTPPSISERGNGSSETTYHPGVLSTLLPHLSPQPDSQTPSAGGADTQTFSSQADNPTLTPAPGGGTDPPGVPGERTVPGTIPADTAFPVDTPSLARNSSAASPTHTSNVSTTDISSGASLTTLTPSTLGLASTDPPSTTIATTTKQTCAAMFGNITVNYTYESSNQTFKADLKDVQNAKCGNEDCENVLNNLEECSQIKNISVSNDSCAPATTIDLYVPPGTDKFSLHDCTPKEKANTSICLEWKTKNLDFRKCNSDNISYVLHCEPENNTKCIRRNTFIPERCQLDNLRAQTNYTCVAEILYRGVKLVKNVINVQTDLGIPETPKPSCGDPAARKTLVSWPEPVSKPESASKPHGYVLCYKNNSEKCKSLPNNVTSFEVESLKPYKYYEVSLLAYVNGKIQRNGTAEKCNFHTKADRPDKVNGMKTSRPTDNSINVTCGPPYETNGPKTFYILVVRSGGSFVTKYNKTNCQFYVDNLYYSTDYEFLVSFHNGVYEGDSVIRNESTNFNAK

• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 145 kDa
• GenBank Accession Number: NM_001111316.2
• Gene Symbol: CD45
• Gene ID (NCBI): 19264
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P06800-4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.