Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombinant Antibody

CD45RO Uni-rAbTM Recombinant Antibody for IHC, FC
Cat No. 65613-1-MR
Clone No.UCHL1

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IHC, FC

CD45, EC:3.1.3.48, GP180, L CA, LCA

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:600-1:2400
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65613-1-MR targets CD45RO in IHC, FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen IL-2 dependent T cell line, CA1 Predict reactive species
Full Name protein tyrosine phosphatase, receptor type, C
GenBank Accession NumberBC014239
Gene Symbol CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_3670360
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).

Protocols

Product Specific Protocols
IHC protocol for CD45RO antibody 65613-1-MRDownload protocol
FC protocol for CD45RO antibody 65613-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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