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- KD/KO Validated
CD47 Monoclonal antibody
CD47 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human and More (2)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1E1D8
Cat no : 66304-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | SKOV-3 cells, A549 cells, HT-1080 cells, Jurkat cells, NCI-H1299 cells, HUVEC cells, K-562 cells, TF-1 cells, human placenta tissue, HeLa cells, HaCaT cells, PANC-1 cells, MDA-MB-231 cells |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 12 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
| FC | See 1 publications below |
Product Information
66304-1-Ig targets CD47 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD47 fusion protein Ag14132 |
| Full Name | CD47 molecule |
| Calculated Molecular Weight | 323 aa, 35 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC010016 |
| Gene Symbol | CD47 |
| Gene ID (NCBI) | 961 |
| RRID | AB_2881687 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD47 antibody 66304-1-Ig | Download protocol |
| IF protocol for CD47 antibody 66304-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Redox Biol Endothelial transferrin receptor 1 contributes to thrombogenesis through cascade ferroptosis | ||
J Control Release Erythrocyte-biomimetic nanosystems to improve antitumor effects of paclitaxel on epithelial cancers. | ||
J Nanobiotechnology Bone-targeted erythrocyte-cancer hybrid membrane-camouflaged nanoparticles for enhancing photothermal and hypoxia-activated chemotherapy of bone invasion by OSCC. | ||
RSC Adv Seedless preparation of Au nanorods by hydroquinone assistant and red blood cell membrane camouflage | ||
J Cell Commun Signal CD47 interactions with exportin-1 limit the targeting of m 7 G-modified RNAs to extracellular vesicles | ||
J Cancer Res Clin Oncol B7-H3 and CD47 co-expression in gastric cancer is a predictor of poor prognosis and potential targets for future dual-targeting immunotherapy |
