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CD47 Monoclonal antibody

CD47 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1E1D8

Cat no : 66304-1-Ig

Synonyms

CD47, CD47 molecule, IAP, Integrin associated protein, Leukocyte surface antigen CD47, MER6, OA3, Protein MER6



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, human placenta tissue, HT-1080 cells, A549 cells, NCI-H1299 cells, SKOV-3 cells, HUVEC cells, TF-1 cells
Positive FC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66304-1-Ig targets CD47 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD47 fusion protein Ag14132
Full Name CD47 molecule
Calculated Molecular Weight 323 aa, 35 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC010016
Gene Symbol CD47
Gene ID (NCBI) 961
RRIDAB_2881687
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.

Protocols

Product Specific Protocols
WB protocol for CD47 antibody 66304-1-IgDownload protocol
FC protocol for CD47 antibody 66304-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,ratWB,IF

Redox Biol

Endothelial transferrin receptor 1 contributes to thrombogenesis through cascade ferroptosis

Authors - Haotian Ma
human,mouseWB

J Control Release

Erythrocyte-biomimetic nanosystems to improve antitumor effects of paclitaxel on epithelial cancers.

Authors - Mingming Song
mouseWB

J Nanobiotechnology

Bone-targeted erythrocyte-cancer hybrid membrane-camouflaged nanoparticles for enhancing photothermal and hypoxia-activated chemotherapy of bone invasion by OSCC.

Authors - Hongying Chen
humanWB

RSC Adv

Seedless preparation of Au nanorods by hydroquinone assistant and red blood cell membrane camouflage

Authors - Jing Li
humanIF,FC

J Cell Commun Signal

CD47 interactions with exportin-1 limit the targeting of m 7 G-modified RNAs to extracellular vesicles

Authors - Sukhbir Kaur
humanWB

J Cancer Res Clin Oncol

B7-H3 and CD47 co-expression in gastric cancer is a predictor of poor prognosis and potential targets for future dual-targeting immunotherapy

Authors - Siji Chen