• Featured Product
  • KD/KO Validated

CD47 Monoclonal antibody

CD47 Monoclonal Antibody for FC, WB,ELISA

Host / Isotype

Mouse / IgG2b


human and More (1)







Cat no : 66304-1-Ig


CD47, CD47 molecule, IAP, Integrin associated protein, Leukocyte surface antigen CD47, MER6, OA3, Protein MER6

Tested Applications

Positive WB detected inHeLa cells, A549 cells, HT-1080 cells, human placenta tissue, HUVEC cells, Jurkat cells, K-562 cells, SKOV-3 cells, NCI-H1299 cells
Positive FC detected inHUVEC cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Sample-dependent, check data in validation data gallery

Product Information

66304-1-Ig targets CD47 in WB, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD47 fusion protein Ag14132
Full Name CD47 molecule
Calculated molecular weight 323 aa, 35 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC010016
Gene symbol CD47
Gene ID (NCBI) 961
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.


Product Specific Protocols
WB protocol for CD47 antibody 66304-1-IgDownload protocol
FC protocol for CD47 antibody 66304-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Clin Lab Anal

MicroRNA-148a-3p inhibits cancer progression and is a novel screening biomarker for gastric cancer.

Authors - Chenhui Bao

J Cell Commun Signal

CD47 interactions with exportin-1 limit the targeting of m 7 G-modified RNAs to extracellular vesicles

Authors - Sukhbir Kaur
  • KD Validated

J Nanobiotechnology

Bone-targeted erythrocyte-cancer hybrid membrane-camouflaged nanoparticles for enhancing photothermal and hypoxia-activated chemotherapy of bone invasion by OSCC.

Authors - Hongying Chen

J Control Release

Erythrocyte-biomimetic nanosystems to improve antitumor effects of paclitaxel on epithelial cancers.

Authors - Mingming Song