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CD47 Monoclonal antibody

CD47 Monoclonal Antibody for FC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, FC,ELISA

Conjugate

Unconjugated

CloneNo.

1E1D8

Cat no : 66304-1-Ig

Synonyms

CD47, CD47 molecule, IAP, Integrin associated protein, Leukocyte surface antigen CD47, MER6, OA3, Protein MER6



Tested Applications

Positive WB detected inHeLa cells, A549 cells, HT-1080 cells, human placenta tissue, HUVEC cells, Jurkat cells, K-562 cells, SKOV-3 cells, NCI-H1299 cells
Positive FC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66304-1-Ig targets CD47 in WB, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD47 fusion protein Ag14132
Full Name CD47 molecule
Calculated molecular weight 323 aa, 35 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC010016
Gene symbol CD47
Gene ID (NCBI) 961
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.

Protocols

Product Specific Protocols
WB protocol for CD47 antibody 66304-1-IgDownload protocol
FC protocol for CD47 antibody 66304-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Clin Lab Anal

MicroRNA-148a-3p inhibits cancer progression and is a novel screening biomarker for gastric cancer.

Authors - Chenhui Bao