Validation Data Gallery
|Positive WB detected in||HeLa cells, A549 cells, HT-1080 cells, human placenta tissue, HUVEC cells, Jurkat cells, K-562 cells, SKOV-3 cells, NCI-H1299 cells|
|Positive FC detected in||HUVEC cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Sample-dependent, check data in validation data gallery|
66304-1-Ig targets CD47 in WB, IF, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||CD47 fusion protein Ag14132|
|Full Name||CD47 molecule|
|Calculated molecular weight||323 aa, 35 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||BC010016|
|Gene ID (NCBI)||961|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.
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