CD47 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CD47 Monoclonal Antibody for WB, FC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E1D8

Cat no : 66304-1-PBS

Synonyms

CD47, CD47 molecule, IAP, Integrin associated protein, Leukocyte surface antigen CD47, MER6, OA3, Protein MER6

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Validation Data Gallery

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  WB analysis using 66304-1-Ig (same clone as 66304-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66304-1-Ig (same clone as 66304-1-PBS)

  WB result of CD47 antibody (66304-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD47 transfected A549 cells. This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66304-1-Ig (same clone as 66304-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66304-1-Ig (same clone as 66304-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66304-1-Ig (same clone as 66304-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 66304-1-Ig (same clone as 66304-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66304-1-Ig (same clone as 66304-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HUVEC using 66304-1-Ig (same clone as 66304-1-PBS)

  1X10^6 HUVEC cells were stained with 0.20 ug Anti-Human CD47 (66304-1-Ig, Clone:1E1D8) (red) or 0.20 ug isotype control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution of 1:1000. Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66304-1-PBS targets CD47 in WB, FC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD47 fusion protein Ag14132
• Full Name: CD47 molecule
• Calculated Molecular Weight: 323 aa, 35 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC010016
• Gene Symbol: CD47
• Gene ID (NCBI): 961
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.