Mouse CD5 Recombinant antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP00448-2

  Sandwich ELISA standard curve of MP00448-2, Mouse CD5 Recombinant Matched Antibody Pair - PBS only. 83451-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0899. 83451-2-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

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  Sample test of MP00448-2

  The mean CD5 concentration was determined to be 502.9 ng/mL in EL-4 cell extract based on a 5.2 mg/mL extract load, 111.7 ng/mL in mouse spleen tissue extract based on a 4.8 mg/mL extract load and 314.2 ng/mL in mouse thymus tissue extract based on a 6 mg/mL extract load.

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  Cytometric bead array standard curve of MP00448-1

  Cytometric bead array standard curve of MP00448-1, MOUSE CD5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83451-1-PBS. Detection antibody: 83451-3-PBS. Standard: Eg0899. Range: 0.156-20 ng/mL.

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  Cytometric bead array standard curve of MP00448-2

  Cytometric bead array standard curve of MP00448-2, MOUSE CD5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83451-1-PBS. Detection antibody: 83451-2-PBS. Standard: Eg0899. Range: 0.156-20 ng/mL.

CD5 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83451-1-PBS

Clone No.240244C4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD5 antigen, Ly 1, Ly 12, Ly A, Lyt 1

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Product Information

83451-1-PBS targets CD5 as part of a matched antibody pair:

MP00448-1: 83451-1-PBS capture and 83451-3-PBS detection (validated in Cytometric bead array)

MP00448-2: 83451-1-PBS capture and 83451-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD5 fusion protein Eg0899
• Full Name: CD5 antigen
• Calculated Molecular Weight: 54 kDa
• GenBank Accession Number: NM_007650.3
• Gene Symbol: Cd5
• Gene ID (NCBI): 12507
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13379
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).