Recombinant Mouse CD5 protein (His Tag)(HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

His Tag

Activity

not tested

Cat no : Eg0899

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Validation Data Gallery

• 

  Purity of Recombinant Mouse CD5 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Mouse CD5 was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse CD5 protein Gln24-Asn370 (Accession# P13379) with a His tag at the C-terminus.
• GeneID: 12507
• Accession: P13379
• PredictedSize: 38.9 kDa
• SDS-PAGE: 45-55 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family. CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells. CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development.

References:

1. J A Ledbetter, et al. (1980) J Exp Med. 152(2):280-95.
2. G S Wood, et al. (1992) Am J Pathol. 141(4):789-95.
3. A Tarakhovsky, et al. (1995) Science. 269(5223):535-7.
4. H S Azzam, et al. (1998) J Exp Med. 188(12):2301-11.
5. P Youinou, et al. (1999) Immunol Today. 20(7):312-6.
6. Chander Raman, et al. (2002) Immunol Res. 26(1-3):255-63.