CD68 Monoclonal antibody

CD68 Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3A9A7

Cat no : 66231-2-Ig

Synonyms

CD68, CD68 molecule, GP110, Macrosialin, SCARD1



Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human lung cancer tissue, human liver cancer tissue, human urothelial carcinoma tissue, human appendicitis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:3000-1:12000
Sample-dependent, check data in validation data gallery

Product Information

66231-2-Ig targets CD68 in IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD68 fusion protein Ag22815
Full Name CD68 molecule
Calculated molecular weight 37 kDa
GenBank accession numberBC015557
Gene symbol CD68
Gene ID (NCBI) 968
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Protocols

Product Specific Protocols
IHC protocol for CD68 antibody 66231-2-IgDownload protocol
IF protocol for CD68 antibody 66231-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

World J Surg Oncol

The downregulation of NCXs is positively correlated with the prognosis of stage II-IV colon cancer.

Authors - Zhixiu Xia
mouseWB,IHC

Int Immunopharmacol

Saikosaponin-d attenuated lipopolysaccharide-induced depressive-like behaviors via inhibiting microglia activation and neuroinflammation.

Authors - Jing Su
humanIHC

Gynecol Oncol

Increased co-expression of MEST and BRCA1 is associated with worse prognosis and immune infiltration in ovarian cancer.

Authors - Jing Zhang
mouseWB

Ecotoxicol Environ Saf

PM2.5-induced lung injury is attenuated in macrophage-specific NLRP3 deficient mice.

Authors - Rui Xiong

Biochem Pharmacol

Berberine exerts anti-tumor activity in diffuse large B-cell lymphoma by modulating c-myc/CD47 axis.

Authors - Shuai Ren
humanIF

Front Immunol

YTHDF1 Negatively Regulates Treponema pallidum-Induced Inflammation in THP-1 Macrophages by Promoting SOCS3 Translation in an m6A-Dependent Manner.

Authors - Zhijia Li