CD68 Monoclonal antibody
CD68 Monoclonal Antibody for IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
3A9A7
Cat no : 66231-2-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human tonsillitis tissue, human lung cancer tissue, human liver cancer tissue, human urothelial carcinoma tissue, human appendicitis tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human tonsillitis tissue |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:1000-1:6000 |
Immunofluorescence (IF) | IF : 1:3000-1:12000 |
Sample-dependent, check data in validation data gallery |
Published Applications
IHC | See 15 publications below |
IF | See 19 publications below |
FC | See 2 publications below |
Product Information
66231-2-Ig targets CD68 in IHC, IF, FC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD68 fusion protein Ag22815 |
Full Name | CD68 molecule |
Calculated molecular weight | 37 kDa |
GenBank accession number | BC015557 |
Gene symbol | CD68 |
Gene ID (NCBI) | 968 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.
Protocols
Product Specific Protocols | |
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IHC protocol for CD68 antibody 66231-2-Ig | Download protocol |
IF protocol for CD68 antibody 66231-2-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Clin Invest Lactate inhibits ATP6V0d2 expression in tumor-associated macrophages to promote HIF-2α-mediated tumor progression. | ||
Redox Biol Redox-sensitive activation of CCL7 by BRG1 in hepatocytes during liver injury. | ||
Transl Res Purinergic receptor P2 × 7 contributes to abdominal aortic aneurysm development via modulating macrophage pyroptosis and inflammation | ||
Cancer Lett SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancer. | ||
Carbohydr Polym Multifunctional chitin-based hollow nerve conduit for peripheral nerve regeneration and neuroma inhibition. | ||
Biochim Biophys Acta Mol Basis Dis Dectin1 contributes to hypertensive vascular injury by promoting macrophage infiltration through activating the Syk/NF-κB pathway |