CoraLite® Plus 488 Anti-Human CD68 (KP1)

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  FC experiment of human PBMCs using CL488-65202

  1X10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 488 Anti-Human CD68 (CL488-65202, Clone:KP1) (red) or unstained (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of human PBMCs using CL488-65202

  1X10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 488 Anti-Human CD68 (CL488-65202, Clone:KP1) or unstained. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CD68 Monoclonal Antibody for FC (Intra)

Cat No. CL488-65202

Clone No.KP1

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC (Intra)

Synonyms

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

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Tested Applications

• Positive FC (Intra) detected in: human PBMCs
• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 5 ul per 10^6 cells in 100 μl suspension
• Flow Cytometry (FC): FC :

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65202 targets CD68 in FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Lysosomal fraction of human alveolar macrophages

• Full Name: CD68 molecule
• Calculated Molecular Weight: 37 kDa
• GenBank Accession Number: BC015557
• Gene Symbol: CD68
• Gene ID (NCBI): 968
• RRID: AB_3084165
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P34810
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.