CoraLite® Plus 647 Anti-Human CD73 (AD2) Rabbit Recombinant Antibody

CD73 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

AD2

Cat no : CL647-65500

Synonyms

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73)

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Validation Data Gallery

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  FC experiment of human PBMCs using CL647-65500

  1x10^6 human PBMCs were surface stained with FITC Anti-Human CD3 and 5 ul CoraLite® Plus 647 Anti-Human CD73 Rabbit Recombinant Antibody (CL647-65500, Clone:AD2) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL647-65500

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD73 Rabbit Recombinant Antibody (CL647-65500, Clone:AD2) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL647-65500

  1x10^6 human PBMCs were surface stained with FITC Anti-Human CD3 and 5 ul CoraLite® Plus 647 Anti-Human CD73 Rabbit Recombinant Antibody (CL647-65500, Clone:AD2) or 5 ul CoraLite® Plus 647 Anti-Human CD73 Mouse Monoclonal Antibody (CL647-65162, Clone:AD2). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65500 targets CD73 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Pre-B leukemia cell line
• Full Name: 5'-nucleotidase, ecto (CD73)
• Calculated Molecular Weight: 29 kDa, 63 kDa
• GenBank Accession Number: BC015940
• Gene Symbol: NT5E
• Gene ID (NCBI): 4907
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).