Anti-Rat CD80 (3H5)

CD80 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa









Cat no : 65217-1-Ig


B7 1, Cd80, Cd80 molecule

Tested Applications

Positive FC detected inLPS-stimulated Wistar rat splenocytes

Recommended dilution

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65217-1-Ig targets CD80 in FC applications and shows reactivity with Rat samples.

Tested Reactivity Rat
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen HTLV-1-transformed Lewis-S1 rat T cell line
Full Name Cd80 molecule
Calculated Molecular Weight 36 kDa
GenBank Accession NumberXM_017597885
Gene Symbol Cd80
Gene ID (NCBI) 25408
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)


Product Specific Protocols
FC protocol for CD80 antibody 65217-1-IgDownload protocol
Standard Protocols
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