CD81 Polyclonal antibody

CD81 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 18250-1-AP

Synonyms

CD81, CD81 antigen, CD81 molecule, S5.7, TAPA1, Tetraspanin 28, Tspan 28, TSPAN28



Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18250-1-AP is CD81 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD81 fusion protein Ag13036
Full Name CD81 molecule
Calculated molecular weight 26 kDa
GenBank accession numberBC002978
Gene symbol CD81
Gene ID (NCBI) 975
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD81 (also known as TAPA1or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

Publications

SpeciesApplicationTitle
humanWB,IHC

Med Sci Monit

Increased Expression of CD81 in Breast Cancer Tissue is Associated with Reduced Patient Prognosis and Increased Cell Migration and Proliferation in MDA-MB-231 and MDA-MB-435S Human Breast Cancer Cell Lines In Vitro.

Authors - Ning Zhang
mouseWB

PeerJ

Heatstroke-induced hepatocyte exosomes promote liver injury by activating the NOD-like receptor signaling pathway in mice.

Authors - Yue Li
mouseWB

Immunol Invest

Bovine Milk Exosomes Affect Proliferation and Protect Macrophages against Cisplatin-Induced Cytotoxicity.

Authors - Svjetlana Matic
humanWB

Biomed Microdevices

Incorporation of VSV-G produces fusogenic plasma membrane vesicles capable of efficient transfer of bioactive macromolecules and mitochondria.

Authors - Hao-Peng Lin
humanWB

Front Oncol

Proteomic Profiling of Serum Exosomes From Patients With Metastatic Gastric Cancer.

Authors - Xiao-Qing Ding
humanFC

Cell Rep

IFITM Proteins Restrict HIV-1 Infection by Antagonizing the Envelope Glycoprotein.

Authors - Jingyou Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Marc (Verified Customer) (08-22-2018)

The antibody preformed as desired. The volume of the sample of antibody was low considering prior publications suggested that it be used at 1:250 and I had several blots. It would be good if you offered this item in a larger volume taking into consideration the amount of antibody needed to use in Western Blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: Exosomes