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CD81 Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 18250-1-AP

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Reviews(1)

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Con: 53 μg/150 μl

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Tested Applications

Applications:
WB, IHC, FC, ELISA
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CD81 Antibody IHC human tonsillitis tissue 18250-1-AP

Immunohistochemical of paraffin-embedded human tonsillitis using 18250-1-AP(CD81 antibody) at dilution of 1:100 (under 10x lens)

CD81 Antibody IHC human tonsillitis tissue 18250-1-AP

Immunohistochemical of paraffin-embedded human tonsillitis using 18250-1-AP(CD81 antibody) at dilution of 1:100 (under 40x lens)

Species specificity:
human
Positive IHC detected in:
human tonsillitis tissue
Recommended dilution:
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CD81 fusion protein Ag13036
Full name:
CD81 molecule
Calculated molecular weight:
26 kDa
Observed molecular weight:
GenBank accession number:
BC002978
Gene ID (NCBI):
975
Gene symbol
CD81
Synonyms
CD81, CD81 antigen, CD81 molecule, S5.7, TAPA1, Tetraspanin 28, Tspan 28, TSPAN28

Background

CD81 (also known as TAPA1or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

Customer Reviews

Share your scientific insight on this antibody

CD81 Rabbit Polyclonal antibody

Marc Wagner, 08-22-2018
Applications
Western Blot,
Lot Number
00018916
Primary Antibody Dilution
1:250
Secondary Antibody Dilution
1:2000
Cell/Tissue Type
Exosomes

The antibody preformed as desired. The volume of the sample of antibody was low considering prior publications suggested that it be used at 1:250 and I had several blots. It would be good if you offered this item in a larger volume taking into consideration the amount of antibody needed to use in Western Blot.
Proteintech Response
Thank you, Marc, for your review. We are delighted that you were able to achieve success with this product for Western blotting. We have forwarded your feedback on the titer to our production team and they are now hard at work at generating a higher titer antibody to improve this product.

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