CD83 Recombinant monoclonal antibody

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  WB analysis using 83907-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83907-5-RR (CD83 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 83907-5-RR

  Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 83907-5-RR (CD83 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

CD83 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83907-5-RR

Clone No.252734F1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CD83 antigen, mCD83

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Tested Applications

• Positive WB detected in: Raji cells, mouse spleen tissue, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83907-5-RR targets CD83 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg10435

  Product name: Recombinant mouse CD83 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 10011478 of

  Sequence: \

• Full Name: CD83 antigen
• Observed Molecular Weight: 50 kDa
• Gene Symbol: Cd83
• Gene ID (NCBI): 12522
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O88324
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also be expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928). PNGase F (peptide N-glycosidase F) digestion reduced the 37 kDa and 50 kDa CD83 forms to 28 kDa (PMID: 15320871).