Anti-Human CD9 (MM2/57)

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  FC experiment of human peripheral blood platelets using 65070-1-Ig

  1X10^6 human peripheral blood platelets were surface stained with 0.25 ug Anti-Human CD9 (65070-1-Ig, Clone: MM2/57) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.25 ug mouse IgG2b isotype control and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

CD9 Monoclonal Antibody for FC

Cat No. 65070-1-Ig

Clone No.MM2/57

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC

Synonyms

CD9 molecule, CD9 antigen, BTCC 1, BA2, 5H9 antigen

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Tested Applications

• Positive FC detected in: human peripheral blood platelets

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65070-1-Ig targets CD9 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  N/A

• Full Name: CD9 molecule
• Calculated Molecular Weight: 228 aa, 25 kDa
• GenBank Accession Number: BC011988
• Gene Symbol: CD9
• Gene ID (NCBI): 928
• RRID: AB_2918376
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P21926
• Storage Buffer: Borate buffered saline, pH 8.2.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD9, also known as Tspan-29, p24 or MIC3, is a member of the tetraspanin superfamily (PMID: 1879540). It is expressed on a large variety of hematopoietic and non-hematopoietic cells, such as stromal cells, megakaryocytes, platelets, B and T lymphocytes, dendritic cells, endothelial cells, mast cells, eosinophils, and basophils (PMID: 30356731). CD9 interacts with the integrin family and other membrane proteins, and is involved in cell adhesion, cell motility and tumor metastasis (PMID: 8478605; 21428940; 25805926). Expression of CD9 enhances membrane fusion between muscle cells and support myotube maintenance (PMID:10459022). On oocytes, CD9 is hypothesized to play a role in fertilization of mammals (PMID: 25536312).