PE Anti-Human CD9 (MM2/57)

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  FC experiment of human peripheral blood platelets using PE-65070

  1X10^6 human peripheral blood platelets were surface stained with 10 ul PE Anti-Human CD9 (PE-65070, Clone:MM2/57) (red) or PE-mouse IgG2b isotype control (black). Cells were not fixed.

CD9 Monoclonal Antibody for FC

Cat No. PE-65070

Clone No.MM2/57

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC

Synonyms

5H9, 5H9 antigen, BA2, BTCC 1, CD9 antigen

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Tested Applications

• Positive FC detected in: human peripheral blood platelets

Recommended dilution

• Flow Cytometry (FC): FC : 10 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-65070 targets CD9 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  N/A

• Full Name: CD9 molecule
• Calculated Molecular Weight: 228 aa, 25 kDa
• GenBank Accession Number: BC011988
• Gene Symbol: CD9
• Gene ID (NCBI): 928
• RRID: AB_2883867
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P21926
• Storage Buffer: PBS with 0.1% sodium azide and a stabilizer, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD9, also known as Tspan-29, p24 or MIC3, is a member of the tetraspanin superfamily (PMID: 1879540). It is expressed on a large variety of hematopoietic and non-hematopoietic cells, such as stromal cells, megakaryocytes, platelets, B and T lymphocytes, dendritic cells, endothelial cells, mast cells, eosinophils, and basophils (PMID: 30356731). CD9 interacts with the integrin family and other membrane proteins, and is involved in cell adhesion, cell motility and tumor metastasis (PMID: 8478605; 21428940; 25805926). Expression of CD9 enhances membrane fusion between muscle cells and support myotube maintenance (PMID:10459022). On oocytes, CD9 is hypothesized to play a role in fertilization of mammals (PMID: 25536312).