CD97 Monoclonal antibody

CD97 Monoclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Mouse / IgG2a


Human, Mouse







Cat no : 66972-1-Ig


CD97, CD97 antigen, CD97 molecule, Leukocyte antigen CD97, TM7LN1

Tested Applications

Positive WB detected inK-562 cells, HepG2 cells, HL-60 cells, DC2.4 cells
Positive IF detected inJurkat cells
Positive FC detected inJurkat cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66972-1-Ig targets CD97 in WB, IF, FC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD97 fusion protein Ag27991
Full Name CD97 molecule
Calculated Molecular Weight 722 aa, 79 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC026690
Gene Symbol CD97
Gene ID (NCBI) 976
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).


Product Specific Protocols
WB protocol for CD97 antibody 66972-1-IgDownload protocol
IF protocol for CD97 antibody 66972-1-IgDownload protocol
FC protocol for CD97 antibody 66972-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols