CDC25B Polyclonal antibody

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  WB analysis of mouse liver using 10644-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10644-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10644-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10644-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10644-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10644-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10644-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 10644-1-AP

  IP result of anti-CDC25B (IP:10644-1-AP, 4ug; Detection:10644-1-AP 1:300) with mouse liver tissue lysate 4000ug.

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  IHC staining of human lymphoma using 10644-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10644-1-AP (CDC25B antibody) at dilution of 1:100 (under 10x lens).

CDC25B Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 10644-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Synonyms

CDC25B, CDC25HU2, M phase inducer phosphatase 2

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Tested Applications

• Positive WB detected in: mouse liver tissue, Jurkat cells, mouse brain tissue, Raji cells
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

10644-1-AP targets CDC25B in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0994

  Product name: Recombinant human CDC25B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-300 aa of BC006395

  Sequence: MEVPQPEPAPGSALSPAGVCGGAQRPGHLPGLLLGSHGLLGSPVRAAASSPVTTLTQTMHDLAGLGSETPKSQVGTLLFRSRSRLTHLSLSRRASESSLSSESSESSDAGLCMDSPSPMDPHMAEQTFEQAIQAASRIIRNEQFAIRRFQSMPVRLLGHSPVLRNITNSQAPDGRRKSEAGSGAASSSGEDKENDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDDDAVPPGMESLISAPLVKT

• Full Name: cell division cycle 25 homolog B (S. pombe)
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC006395
• Gene Symbol: CDC25B
• Gene ID (NCBI): 994
• RRID: AB_2075439
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P30305
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. In Jurkat cell, this antibody recognized 2 bands: 60kd(isoform 2, CDC25B2) and 70kd(isoform 4 of CDC25B).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB, IF	BMC Dev Biol; Evidence for the requirement of 14-3-3eta (YWHAH) in meiotic spindle assembly during mouse oocyte maturation.; Authors - Santanu De
mouse	IF	BMC Dev Biol; YWHA (14-3-3) protein isoforms and their interactions with CDC25B phosphatase in mouse oogenesis and oocyte maturation.; Authors - Alaa A Eisa