CDC25B Polyclonal antibody

CDC25B Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 10644-1-AP


CDC25B, CDC25HU2, M phase inducer phosphatase 2

Tested Applications

Positive WB detected inmouse liver tissue, Jurkat cells, mouse brain tissue, Raji cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10644-1-AP targets CDC25B in WB, IP, IHC, IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC25B fusion protein Ag0994
Full Name cell division cycle 25 homolog B (S. pombe)
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC006395
Gene Symbol CDC25B
Gene ID (NCBI) 994
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. In Jurkat cell, this antibody recognized 2 bands: 60kd(isoform 2, CDC25B2) and 70kd(isoform 4 of CDC25B).


Product Specific Protocols
WB protocol for CDC25B antibody 10644-1-APDownload protocol
IHC protocol for CDC25B antibody 10644-1-APDownload protocol
IP protocol for CDC25B antibody 10644-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols


mouseWB, IF

BMC Dev Biol

Evidence for the requirement of 14-3-3eta (YWHAH) in meiotic spindle assembly during mouse oocyte maturation.

Authors - Santanu De

BMC Dev Biol

YWHA (14-3-3) protein isoforms and their interactions with CDC25B phosphatase in mouse oogenesis and oocyte maturation.

Authors - Alaa A Eisa