P-cadherin Polyclonal antibody

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• P-cadherin Polyclonal antibody (13773-1-AP)

• KD/KO Validated

P-cadherin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13773-1-AP Copied!

Publications (20)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Cadherin 3, Cadherin-3, CDH3, CDHP, HJMD

• 

  WB analysis of A431 using 13773-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13773-1-AP (P-cadherin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 13773-1-AP

  WB result of P-cadherin antibody (13773-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P-cadherin transfected A431 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of A431 using 13773-1-AP

  IP result of anti-P-cadherin (IP:13773-1-AP, 4ug; Detection:13773-1-AP 1:300) with A431 cells lysate 3040ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 13773-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13773-1-AP (P-cadherin antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 13773-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13773-1-AP (P-cadherin antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A431 using 13773-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 13773-1-AP (P-cadherin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A431 using 13773-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using P-cadherin antibody (13773-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A431 using 13773-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 13773-1-AP (P-cadherin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13773-1-AP targets P-cadherin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4758

  Product name: Recombinant human CDH3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 24-349 aa of BC041846

  Sequence: SEPCRAVFREAEVTLEAGGAEQEPGQALGKVFMGCPGQEPALFSTDNDDFTVRNGETVQERRSLKERNPLKIFPSKRILRRHKRDWVVAPISVPENGKGPFPQRLNQLKSNKDRDTKIFYSITGPGADSPPEGVFAVEKETGWLLLNKPLDREEIAKYELFGHAVSENGASVEDPMNISIIVTDQNDHKPKFTQDTFRGSVLEGVLPGTSVMQMTATDEDDAIYTYNGVVAYSIHSQEPKDPHDLMFTIHRSTGTISVISSGLDREKVPEYTLTIQATDMDGDGSTTTAVAVVEILDANDNAPMFDPQKYEAHVPENAVGHEVQRL

• Full Name: cadherin 3, type 1, P-cadherin (placental)
• Calculated Molecular Weight: 829 aa, 91 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC041846
• Gene Symbol: P-cadherin
• Gene ID (NCBI): 1001
• RRID: AB_10596468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P22223
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. P-cadherin (placental cadherin), also known as CDH3 (cadherin 3), is a classical member of the cadherin superfamily, which also includes E-, N-, R-, and B-cadherins. Aberrant expression of P-cadherin has been reported in many cancers, including breast, gastric, endometrial, ovarian, and prostate cancer.(PMID: 11171368; 30637694; 26438065;18001487)

Publications

What published studies show

The P-cadherin Polyclonal antibody (Cat# 13773-1-AP) has 20 citations in journals including Gastroenterology, Nature Neuroscience, Nature Communications, Journal of Advanced Research, Science Translational Medicine, International Journal of Biological Sciences, Antioxidants, Clinical and Experimental Hypertension, Journal of Ethnopharmacology, Molecular Therapy Nucleic Acids, Aging, International Immunopharmacology, Cell Signaling, Journal of Inflammation Research, BMC Complementary Medicine and Therapies, BMC Cancer, Translational Lung Cancer Research, Frontiers in Oncology, Journal of Oral Pathology and Medicine, and Developmental Dynamics. Research fields span pancreatic cancer, Alzheimer's disease, diabetic kidney disease, osteoarthritis, hepatoma, lung cancer, colorectal cancer, gastric cancer, oral squamous cell carcinoma, ulcerative colitis, and skin development.

Automatically generated from the 20 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB	Gastroenterology; Cell-in-Cell-Mediated Entosis Reveals A Progressive Mechanism in Pancreatic Cancer; Authors - Jianlu Song
mouse	IF	Nat Neurosci; Spinal cord Tau pathology induces tactile deficits and cognitive impairment in Alzheimer's disease via dysregulation of CCK neurons; Authors - Yang Zhou
mouse	IF	Nat Commun; Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice.; Authors - Hans I-Chen Harn
mouse	WB	J Adv Res; Podocyte TLR4 deletion alleviates diabetic kidney disease through prohibiting PKCδ/SHP-1-dependent ER stress and relieving podocyte damage and inflammation; Authors - Zongze Li
mouse	IHC	Sci Transl Med; Inhibition of a signaling modality within the gp130 receptor enhances tissue regeneration and mitigates osteoarthritis; Authors - Ruzanna Shkhyan
human	WB,IHC	Int J Biol Sci; KLF4-Mediated CDH3 Upregulation Suppresses Human Hepatoma Cell Growth and Migration via GSK-3β Signaling.; Authors - Li Li