P-cadherin Polyclonal antibody

P-cadherin Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 13773-1-AP

Synonyms

Cadherin 3, CDH3, CDHP, HJMD, PCAD, P-cadherin



Tested Applications

Positive WB detected inA431 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman breast cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13773-1-AP targets P-cadherin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P-cadherin fusion protein Ag4758
Full Name cadherin 3, type 1, P-cadherin (placental)
Calculated molecular weight 829 aa, 91 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC041846
Gene symbol CDH3
Gene ID (NCBI) 1001
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. P-cadherin (placental cadherin), also known as CDH3 (cadherin 3), is a classical member of the cadherin superfamily which also include E-, N-, R-, and B-cadherins. Aberrant expression of P-cadherin has been reported in many cancers, including breast, gastric, endometrial, ovarian, and prostate cancer.

Protocols

Product Specific Protocols
WB protocol for P-cadherin antibody 13773-1-APDownload protocol
IHC protocol for P-cadherin antibody 13773-1-APDownload protocol
IF protocol for P-cadherin antibody 13773-1-APDownload protocol
IP protocol for P-cadherin antibody 13773-1-APDownload protocol
FC protocol for P-cadherin antibody 13773-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Clin Exp Hypertens

PMID: 30698033

Authors - Dongdong Sun
humanIHC

Aging (Albany NY)

Transcriptional dysregulation of TRIM29 promotes colorectal cancer carcinogenesis via pyruvate kinase-mediated glucose metabolism.

Authors - Jing Han
mouseWB

BMC Complement Med Ther

Vincetoxicum arnottianum modulates motility features and metastatic marker expression in pediatric rhabdomyosarcoma by stabilizing the actin cytoskeleton.

Authors - Anna Adamus
humanWB,IHC

Int J Biol Sci

KLF4-Mediated CDH3 Upregulation Suppresses Human Hepatoma Cell Growth and Migration via GSK-3β Signaling.

Authors - Li Li
humanWB,IHC

Front Oncol

Screening and Identification of Key Biomarkers of Gastric Cancer: Three Genes Jointly Predict Gastric Cancer.

Authors - Meng-Jie Shan
mouseIF

Nat Commun

Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice.

Authors - Hans I-Chen Harn