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CDK2 Polyclonal antibody
CDK2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
202
Cat no : 10122-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, Jurkat cells, HeLa cells, HL-60 cells, HEK-293 cells, K-562 cells, MCF-7 cells, A431 cells, NIH/3T3 cells, rat kidney tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human liver cancer tissue, human prostate cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, mouse testis tissue |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 4 publications below |
WB | See 198 publications below |
IHC | See 8 publications below |
IF | See 1 publications below |
FC | See 1 publications below |
CoIP | See 3 publications below |
Product Information
10122-1-AP targets CDK2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, fish, sea cucumbers, sheep, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CDK2 fusion protein Ag0166 |
Full Name | cyclin-dependent kinase 2 |
Calculated molecular weight | 33 kDa |
Observed molecular weight | 33 kDa |
GenBank accession number | BC003065 |
Gene symbol | CDK2 |
Gene ID (NCBI) | 1017 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CDK2 antibody 10122-1-AP | Download protocol |
IHC protocol for CDK2 antibody 10122-1-AP | Download protocol |
IF protocol for CDK2 antibody 10122-1-AP | Download protocol |
IP protocol for CDK2 antibody 10122-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Signal Transduct Target Ther SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis
| ||
Mol Cancer LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.
| ||
Nat Commun Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway. | ||
J Clin Invest TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy. | ||
Nat Commun Whole-exome sequencing of alpha-fetoprotein producing gastric carcinoma reveals genomic profile and therapeutic targets. | ||
Nat Commun CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Silvia (Verified Customer) (10-19-2021) | It works well for WB
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