• Featured Product
  • KD/KO Validated

CDK2 Polyclonal antibody

CDK2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10122-1-AP

Synonyms

CDK2, Cell division protein kinase 2, cyclin dependent kinase 2, p33 protein kinase, p33(CDK2)



Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, HeLa cells, HL-60 cells, HEK-293 cells, K-562 cells, MCF-7 cells, A431 cells, NIH/3T3 cells, rat kidney tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, human prostate cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, mouse testis tissue
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10122-1-AP targets CDK2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, fish, sea cucumbers, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDK2 fusion protein Ag0166
Full Name cyclin-dependent kinase 2
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC003065
Gene symbol CDK2
Gene ID (NCBI) 1017
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for CDK2 antibody 10122-1-APDownload protocol
IHC protocol for CDK2 antibody 10122-1-APDownload protocol
IF protocol for CDK2 antibody 10122-1-APDownload protocol
IP protocol for CDK2 antibody 10122-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Signal Transduct Target Ther

SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis

Authors - Saisai Guo
  • KD Validated
humanWB,IHC

Mol Cancer

LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.

Authors - Xi Jin
  • KD Validated
humanWB

Nat Commun

Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.

Authors - Xiaoyu Ma
humanWB

J Clin Invest

TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy.

Authors - Zheng Hu
humanWB

Nat Commun

Whole-exome sequencing of alpha-fetoprotein producing gastric carcinoma reveals genomic profile and therapeutic targets.

Authors - Jun Lu
humanWB

Nat Commun

CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity.

Authors - Youzhou Sang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Silvia (Verified Customer) (10-19-2021)

It works well for WB

  • Applications: Western Blot
  • Cell Tissue Type: Human fibroblasts