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- KD/KO Validated
CDK2 Polyclonal antibody
CDK2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 10122-1-AP
Validation Data Gallery
|Positive WB detected in||HepG2 cells, Jurkat cells, HeLa cells, HL-60 cells, HEK-293 cells, K-562 cells, MCF-7 cells, A431 cells, NIH/3T3 cells, rat kidney tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human liver cancer tissue, human prostate cancer tissue, human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, mouse testis tissue|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10122-1-AP targets CDK2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, fish, sea cucumbers, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CDK2 fusion protein Ag0166|
|Full Name||cyclin-dependent kinase 2|
|Calculated molecular weight||33 kDa|
|Observed molecular weight||33 kDa|
|GenBank accession number||BC003065|
|Gene ID (NCBI)||1017|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.
|Product Specific Protocols|
|WB protocol for CDK2 antibody 10122-1-AP||Download protocol|
|IHC protocol for CDK2 antibody 10122-1-AP||Download protocol|
|IF protocol for CDK2 antibody 10122-1-AP||Download protocol|
|IP protocol for CDK2 antibody 10122-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Signal Transduct Target Ther
SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis
LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.
Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.
J Clin Invest
TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy.
Whole-exome sequencing of alpha-fetoprotein producing gastric carcinoma reveals genomic profile and therapeutic targets.
CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Silvia (Verified Customer) (10-19-2021)
It works well for WB