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- KD/KO Validated
CDK6 Polyclonal antibody
CDK6 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat no : 14052-1-AP
Validation Data Gallery
|Positive WB detected in||Jurkat cells, HeLa cells, C2C12 cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human lung cancer tissue, human endometrial cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14052-1-AP targets CDK6 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CDK6 fusion protein Ag5275|
|Full Name||cyclin-dependent kinase 6|
|Calculated molecular weight||326 aa, 36 kDa|
|Observed molecular weight||36-40 kDa|
|GenBank accession number||BC027989|
|Gene ID (NCBI)||1021|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cyclin-dependent kinase 6 (CDK6) is an enzyme belonging to the CDK family. CDK6 is a catalytic subunit of a
protein kinase complex that is essential for progression through the G1 phase of the cell cycle and G1/S transition,
thus activating cell proliferation. CDK6 partners with cyclins to phosphorylate the retinoblastoma (pRb) protein to
release its binding partner E2F; a transcription factor that drives gene expression necessary for DNA replication
(PMID: 8114739). CDK6 was long thought of as a redundant homolog of CDK4 as they perform similar roles in cell
cycle progression. But recently it has become apparent that CDK6 and CDK4 differ in tissue-specific functions,
where CDK6 plays a role in differentiation (PMID: 16410727) but also in contribution to tumor development.
What is the molecular weight of CDK6?
The molecular weight of CDK6 is approximately 36-40 kDa (PMID: 23563707, 8114739).
What is the subcellular localization of CDK6?
CDK6 is ubiquitously expressed through the nucleus and cytoplasm. However, the most active complexes are
located in the nuclei of proliferating cells.
What is the expression pattern of CDK6?
CDK6 activity first occurs midway through the G1 phase. Its expression is regulated by D-type cyclins and
members of the INK4 family of CDK inhibitors (PMID: 7739547).
What is CDK6's role in cancer?
CDK6 is involved in a critical regulatory point in the cell cycle and so is found to be unbalanced in approximately
80-90% of tumors (PMID: 23356980). Loss of cell cycle control is the initial step in the development of the
hallmarks of cancer, such as sustained proliferative signaling and dysregulated cellular energetics. Deregulation
of CDK6 has an important role in lymphoma by increasing angiogenesis (PMID: 23948297) and upregulated CDK6
expression is also associated with a poor clinical prognosis in medulloblastoma (PMID: 23172372).
|Product Specific Protocols|
|WB protocol for CDK6 antibody 14052-1-AP||Download protocol|
|IHC protocol for CDK6 antibody 14052-1-AP||Download protocol|
|IF protocol for CDK6 antibody 14052-1-AP||Download protocol|
|IP protocol for CDK6 antibody 14052-1-AP||Download protocol|
|FC protocol for CDK6 antibody 14052-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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