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  • KD/KO Validated

CDK6 Antibody

CDK6 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14052-1-AP

Synonyms

CDK6, Cell division protein kinase 6, cyclin dependent kinase 6, PLSTIRE



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, C2C12 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman lung cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14052-1-AP targets CDK6 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDK6 fusion protein Ag5275
Full Name cyclin-dependent kinase 6
Calculated molecular weight 326aa,36 kDa
Observed molecular weight 36-40 kDa
GenBank accession number BC027989
Gene symbol CDK6
Gene ID (NCBI) 1021
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin-dependent kinase 6 (CDK6) is an enzyme belonging to the CDK family. CDK6 is a catalytic subunit of a 

protein kinase complex that is essential for progression through the G1 phase of the cell cycle and G1/S transition, 

thus activating cell proliferation. CDK6 partners with cyclins to phosphorylate the retinoblastoma (pRb) protein to 

release its binding partner E2F; a transcription factor that drives gene expression necessary for DNA replication 

(PMID: 8114739). CDK6 was long thought of as a redundant homolog of CDK4 as they perform similar roles in cell 

cycle progression. But recently it has become apparent that CDK6 and CDK4 differ in tissue-specific functions, 

where CDK6 plays a role in differentiation (PMID: 16410727) but also in contribution to tumor development.


What is the molecular weight of CDK6?

The molecular weight of CDK6 is approximately 36–40 kDa (PMID: 23563707, 8114739).


What is the subcellular localization of CDK6?

CDK6 is ubiquitously expressed through the nucleus and cytoplasm. However, the most active complexes are 

located in the nuclei of proliferating cells.


What is the expression pattern of CDK6?

CDK6 activity first occurs midway through the G1 phase. Its expression is regulated by D-type cyclins and 

members of the INK4 family of CDK inhibitors (PMID: 7739547).


What is CDK6’s role in cancer?

CDK6 is involved in a critical regulatory point in the cell cycle and so is found to be unbalanced in approximately 

80–90% of tumors (PMID: 23356980). Loss of cell cycle control is the initial step in the development of the 

hallmarks of cancer, such as sustained proliferative signaling and dysregulated cellular energetics. Deregulation 

of CDK6 has an important role in lymphoma by increasing angiogenesis (PMID: 23948297) and upregulated CDK6 

expression is also associated with a poor clinical prognosis in medulloblastoma (PMID: 23172372).


Protocols

Product Specific Protocols
WB protocol for CDK6 antibody 14052-1-APDownload protocol
IHC protocol for CDK6 antibody 14052-1-APDownload protocol
IF protocol for CDK6 antibody 14052-1-APDownload protocol
IP protocol for CDK6 antibody 14052-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cancer

Long non-coding RNA PVT1 promotes tumor progression by regulating the miR-143/HK2 axis in gallbladder cancer.

Authors - Jianan Chen
humanWB

BMC Cancer

Short-term stimulation with histone deacetylase inhibitor trichostatin a induces epithelial-mesenchymal transition in nasopharyngeal carcinoma cells without increasing cell invasion ability.

Authors - Zhihua Shen
humanWB

J Enzyme Inhib Med Chem

Design, synthesis and molecular mechanisms of novel dual inhibitors of heat shock protein 90/phosphoinositide 3-kinase alpha (Hsp90/PI3Kα) against cutaneous melanoma.

Authors - Feifei Qin
humanWB

PLoS Biol

G1/S cell cycle regulators mediate effects of circadian dysregulation on tumor growth and provide targets for timed anticancer treatment.

Authors - Yool Lee
WB

Exp Eye Res

Fibronectin regulates the self-renewal of rabbit limbal epithelial stem cells by stimulating the Wnt11/Fzd7/ROCK non-canonical Wnt pathway.

Authors - Mingyue Zheng
humanWB

J Exp Clin Cancer Res

IPO5 promotes the proliferation and tumourigenicity of colorectal cancer cells by mediating RASAL2 nuclear transportation.

Authors - Wenjuan Zhang