CDR2 Polyclonal antibody
CDR2 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
WB, IP, IHC, ELISA
Cat no : 11611-2-AP
|Positive WB detected in||human brain tissue, Y79 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11611-2-AP targets CDR2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CDR2 fusion protein Ag2183|
|Full Name||cerebellar degeneration-related protein 2, 62kDa|
|Calculated molecular weight||454 aa, 52 kDa|
|Observed molecular weight||62 kDa|
|GenBank accession number||BC017503|
|Gene ID (NCBI)||1039|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Patients with paraneoplastic cerebellar degeneration (PCD) carry a characteristic antibody called anti-Yo. On Western blot analysis of Purkinje cells and tumor tissue from CD patient, the anti-Yo sera react with at least 2 antigens, a major species of 62 kD called CDR62 or CDR2 [PMID:18045792]. CDR2 is partly characterized, as CDR2 through its leucine zipper motif has been demonstrated to interact with c-myc, with cell cycle-related proteins and with a protein kinase, indicating that CDR2 is involved in signal transduction and gene transcription. Furthermore, CDR2 has been found to attenuate hypoxic response in renal cell carcinoma, and recent data suggest a role for CDR2 and c-myc in mitosis in cycling cells [PMID:21080165].
|Product Specific Protocols|
|WB protocol for CDR2 antibody 11611-2-AP||Download protocol|
|IHC protocol for CDR2 antibody 11611-2-AP||Download protocol|
|Click here to view our Standard Protocols|
Paraneoplastic CDR2 and CDR2L antibodies affect Purkinje cell calcium homeostasis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
anwesh (Verified Customer) (11-11-2019)
CDR2 was successfully IP'ed from A549 cells. The antibody had not background bands.
anwesh (Verified Customer) (07-29-2019)
The protein was found at the right size and no additional background bands were observed around the size range. Perfect!