CDR2 Polyclonal antibody

CDR2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (2)

Cat no : 11611-2-AP

Synonyms

CDR2, CDR62, PCD17, Yo

---

Validation Data Gallery

• 

  WB analysis of human brain using 11611-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11611-2-AP (CDR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11611-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11611-2-AP (CDR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 11611-2-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 11611-2-AP (CDR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 11611-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11611-2-AP (CDR2 antibody) at dilution of 1:50 (under 10x lens).

---

Tested Applications

• Positive WB detected in: human brain tissue, Y79 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IP: See 1 publications below

Product Information

11611-2-AP targets CDR2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDR2 fusion protein Ag2183
• Full Name: cerebellar degeneration-related protein 2, 62kDa
• Calculated molecular weight: 454 aa, 52 kDa
• Observed molecular weight: 62 kDa
• GenBank accession number: BC017503
• Gene symbol: CDR2
• Gene ID (NCBI): 1039
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Patients with paraneoplastic cerebellar degeneration (PCD) carry a characteristic antibody called anti-Yo. On Western blot analysis of Purkinje cells and tumor tissue from CD patient, the anti-Yo sera react with at least 2 antigens, a major species of 62 kD called CDR62 or CDR2 [PMID:18045792]. CDR2 is partly characterized, as CDR2 through its leucine zipper motif has been demonstrated to interact with c-myc, with cell cycle-related proteins and with a protein kinase, indicating that CDR2 is involved in signal transduction and gene transcription. Furthermore, CDR2 has been found to attenuate hypoxic response in renal cell carcinoma, and recent data suggest a role for CDR2 and c-myc in mitosis in cycling cells [PMID:21080165].

Publications

Species	Application	Title
human	IP, WB	Acta Neuropathol; Paraneoplastic CDR2 and CDR2L antibodies affect Purkinje cell calcium homeostasis.; Authors - Manja Schubert

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

anwesh (Verified Customer) (11-11-2019)

CDR2 was successfully IP'ed from A549 cells. The antibody had not background bands.

• Applications: Immunoprecipitation,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: A594 cells

FH

anwesh (Verified Customer) (07-29-2019)

The protein was found at the right size and no additional background bands were observed around the size range. Perfect!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T, U2OS, HaCaT

CDR2 Antibody Western Blot, validation (1:1000 dilution) in 293T, U2OS, HaCaT (Cat no:11611-2-AP)