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CEA Antibody

CEA Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, chIP, ELISA

Conjugate

Unconjugated

Cat no : 10421-1-AP

Synonyms

Carcinoembryonic antigen, CD66e, CEA, CEACAM5, Meconium antigen 100



Tested Applications

Positive IHC detected inhuman colon cancer tissue, human stomach cancer tissue, human appendicitis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10421-1-AP targets CEA in WB, IHC, chIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEA fusion protein Ag0672
Full Name carcinoembryonic antigen-related cell adhesion molecule 5
Calculated molecular weight 77 kDa
Observed molecular weight
GenBank accession number BC034671
Gene symbol CEACAM5
Gene ID (NCBI) 1048
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.

Protocols

Product Specific Protocols
IHC protocol for CEA antibody 10421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Gene Ther

Lentivirus-mediated RASSF1A expression suppresses aggressive phenotypes of gastric cancer cells in vitro and in vivo.

Authors - P-H Zhou
humanWB

Transl Res

Molecular signature of interleukin-22 in colon carcinoma cells and organoid models.

Authors - Ina Rudloff
humanIHC

World Neurosurg

Discovery of Aquaporin-1 and Aquaporin-4 Expression in an Intramedullary Spinal Cord Ependymal Cyst: Case Report.

Authors - Matthew J Shepard
humanWB,IHC,chIP

Int J Biol Sci

FBW7 suppresses metastasis of colorectal cancer by inhibiting HIF1α/CEACAM5 functional axis.

Authors - Qingguo Li
  • KD Validated