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CEA Polyclonal antibody
CEA Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF-P, chIP, ELISA
Conjugate
Unconjugated
Cat no : 10421-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HT-29 cells, MCF-7 cells, human saliva |
Positive IHC detected in | human colon cancer tissue, human appendicitis tissue, human stomach cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human colon cancer tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 4 publications below |
IHC | See 4 publications below |
ChIP | See 1 publications below |
Product Information
10421-1-AP targets CEA in WB, IHC, IF-P, chIP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CEA fusion protein Ag0672 |
Full Name | carcinoembryonic antigen-related cell adhesion molecule 5 |
Calculated Molecular Weight | 77 kDa |
Observed Molecular Weight | 180-200 kDa, 77 kDa |
GenBank Accession Number | BC034671 |
Gene Symbol | CEA |
Gene ID (NCBI) | 1048 |
RRID | AB_2244691 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.
Protocols
Product Specific Protocols | |
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WB protocol for CEA antibody 10421-1-AP | Download protocol |
IHC protocol for CEA antibody 10421-1-AP | Download protocol |
IF protocol for CEA antibody 10421-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Transl Res Molecular signature of interleukin-22 in colon carcinoma cells and organoid models. | ||
Int J Biol Sci FBW7 suppresses metastasis of colorectal cancer by inhibiting HIF1α/CEACAM5 functional axis.
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Gene Ther Lentivirus-mediated RASSF1A expression suppresses aggressive phenotypes of gastric cancer cells in vitro and in vivo. | ||
World Neurosurg Discovery of Aquaporin-1 and Aquaporin-4 Expression in an Intramedullary Spinal Cord Ependymal Cyst: Case Report. | ||
PLoS One Correlation of alpha-1 antitrypsin levels and exosome associated neutrophil elastase endothelial injury in subjects with SARS-CoV2 infection | ||
Iran J Basic Med Sci Preparation and application of patient-derived xenograft mice model of colorectal cancer |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Claire (Verified Customer) (09-30-2024) | A repeat order of an antibody previously used in colorectal adenocarcinoma research as this produced excellent images previously, we are now aiming to optimise this antibody for pancreatic work
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