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CEA Polyclonal antibody

CEA Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, chIP, ELISA

Conjugate

Unconjugated

Cat no : 10421-1-AP

Synonyms

Carcinoembryonic antigen, CD66e, CEA, CEACAM5, Meconium antigen 100



Tested Applications

Positive IHC detected inhuman colon cancer tissue, human stomach cancer tissue, human appendicitis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10421-1-AP targets CEA in WB, IHC, chIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEA fusion protein Ag0672
Full Name carcinoembryonic antigen-related cell adhesion molecule 5
Calculated molecular weight 77 kDa
GenBank accession numberBC034671
Gene symbol CEACAM5
Gene ID (NCBI) 1048
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.

Protocols

Product Specific Protocols
IHC protocol for CEA antibody 10421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Gene Ther

Lentivirus-mediated RASSF1A expression suppresses aggressive phenotypes of gastric cancer cells in vitro and in vivo.

Authors - P-H Zhou
humanWB

Transl Res

Molecular signature of interleukin-22 in colon carcinoma cells and organoid models.

Authors - Ina Rudloff
humanIHC

World Neurosurg

Discovery of Aquaporin-1 and Aquaporin-4 Expression in an Intramedullary Spinal Cord Ependymal Cyst: Case Report.

Authors - Matthew J Shepard
humanWB,IHC,chIP

Int J Biol Sci

FBW7 suppresses metastasis of colorectal cancer by inhibiting HIF1α/CEACAM5 functional axis.

Authors - Qingguo Li
  • KD Validated